Results 31 to 40 of about 95,299 (308)

A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

BMC Research Notes, 2012
Background We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments.
Dimauro Ivan, Pearson Timothy, Caporossi Daniela, Jackson Malcolm J   +3 more
doaj   +1 more source

Calcium uptake by subcellular fractions of human umbilical artery

, 1976
Two different mechanisms for the active accumulation of Ca2+ by subcellular fractions of human umbilical artery are described. One, located in the mitochondrial fraction, was induced by exogenous ATP or respiratory substrates (ADP and succinate) and was
CJ Lovell-Smith, RI Clyman, VC Manganiello, M Vaughan   +3 more
core   +1 more source

Exploring Subcellular Cerebellar Fractions with the Electron Microscope

, 2021
Differential ultracentrifugation and subcellular fractionation historically helped to study the components of the cell, to discover new cellular organelles, and to decipher their morphological and molecular properties.
Triarhou, Lazaros, Manto, Mario
core   +1 more source

HPLC assay of zearalenone and reduced metabolites in S9 fractions of duck liver [PDF]

, 2007
HPLC analysis of zearalenone (ZEA), zearalenols (-ZOL and ß-ZOL) and zearalanols (-ZAL and ß-ZAL) was developed, in order to obtain a sensitive and reproducible method to quantify ZEA and its reduced metabolites in subcellular fractions of animal livers (
Guerre, Philippe, Kolf-Clauw, Martine, Tardieu, Didier, Ayouni, Fatma   +3 more
core  

Proteolytic activity and quantity of legumain in subcellular fractions of HCT116 and SW620 cells.

, 2013
(A) Proteolytic activity of legumain determined by substrate cleavage (Z-Ala-Ala-Asn-NHMec) relative to total protein content of each subcellular compartment of HCT116 (dotted bars) and SW620 (chequered bars) cells after density gradient centrifugations.
Kjersti Flatmark (76807), Solveig J. Pettersen (103826), Harald T. Johansen (103823), Rigmor Solberg (103828), Klaudia Brix (83269), Mads H. Haugen (103820), Gunhild M. Maelandsmo (103830)   +6 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Comparative proteomics of two Mycoplasma hyopneumoniae strains and Mycoplasma flocculare identified potential porcine enzootic pneumonia determinants

Virulence, 2018
Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia
Jéssica Andrade Paes, Lais Del Prá Netto Machado, Fernanda Munhoz dos Anjos Leal, Sofia Nóbrega De Moraes, Hercules Moura, John R. Barr, Henrique Bunselmeyer Ferreira   +6 more
doaj   +1 more source

The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures

Journal of Lipid Research, 1966
The roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-14C (0.005%) supplemented with cholestanol-7α-3H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ...
N.L. Lasser, R.B. Clayton
doaj   +1 more source

Biological significance of metals partitioned to subcellular fractions within earthworms (Aporrectodea caliginosa).

, 2006
Metal ions in excess of metabolic requirements are potentially toxic and must be removed from the vicinity of important biological molecules to protect organisms from adverse effects.
van Straalen, N.M., Martina G. Vijver, Nico M. van Straalen, Straalen, Nico M van, Cornelis A. M. van Gestel, Peijnenburg, Willie J G M, Peijnenburg, W., Roman P. Lanno, Vijver, M.G., Lanno, Roman P, Willie J. G. M. Peijnenburg, Vijver, Martina G, van Gestel, C.A.M., Gestel, Cornelis A M van, Lanno, R.P., van Gestel, C.A.M.; id_orcid   +15 more
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source