Preparation of Crude Subcellular Fractions by Differential Centrifugation
The Scientific World Journal, 2002 The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
doaj +1 more source
Lipid composition of subcellular particles of human blood platelets
Journal of Lipid Research, 1969 Human platelets can be fractionated into three main subcellular components: granules, membranes, and a soluble fraction. In this study we determined the phospholipid and neutral lipid content of the granules and membranes.
Aaron J. Marcus +2 more
doaj +1 more source
Journal of Lipid Research, 1985 During myocardial ischemia increased levels of lysoglycerophospholipids have been reported which may be deleterious to myocardial function. Phospholipases are presumed to be important in the regulation of this process.
G Nalbone, K Y Hostetler
doaj +1 more source
De novo synthesis and elongation of fatty acids by subcellular fractions of monkey aorta
Journal of Lipid Research, 1968 Subcellular fractions of aorta of squirrel monkey (Saimiri sciureus) were examined for their ability to synthesize and elongate fatty acids. High-speed supernate (HSS) incorporated substantial quantities of malonyl CoA into fatty acids while acetyl CoA ...
Charles F. Howard, Jr.
doaj +1 more source
Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients [PDF]
, 1984 Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes ...
Bowers +31 more
core +1 more source
Protein synthesis at synaptic sites on dendrites [PDF]
, 2001 Studies over the past 20 years have revealed that gene expression in neurons is carried out by a distributed network of translational machinery. One component of this network is localized in dendrites, where polyribosomes and associated membranous ...
Schuman, Erin M., Steward, Oswald
core +1 more source
Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex [PDF]
, 2014 Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of
Alan R. Prescott +60 more
core +3 more sources
A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
BMC Research Notes, 2012 Background We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments.
Dimauro Ivan +3 more
doaj +1 more source
Alterations in the mitochondrial proteome of neuroblastoma cells 2 in response to complex 1 inhibition [PDF]
, 2011 Increasing evidence points to mitochondrial dysfunction in Parkinson's disease (PD) associated with complex I dysfunction, but the exact pathways which lead to cell death have not been resolved.
Billett, EE +3 more
core +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source