Results 31 to 40 of about 511,662 (296)
Journal of Lipid Research, 1985 During myocardial ischemia increased levels of lysoglycerophospholipids have been reported which may be deleterious to myocardial function. Phospholipases are presumed to be important in the regulation of this process.
G Nalbone, K Y Hostetler
doaj +1 more source
A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
BMC Research Notes, 2012 Background We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments.
Dimauro Ivan +3 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane. [PDF]
FEBS Open BioAn in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Ghosh S, Mukherjee K, Bhattacharyya SN.
europepmc +2 more sources
HPLC assay of zearalenone and reduced metabolites in S9 fractions of duck liver [PDF]
, 2007 HPLC analysis of zearalenone (ZEA), zearalenols (-ZOL and ß-ZOL) and zearalanols (-ZAL and ß-ZAL) was developed, in order to obtain a sensitive and reproducible method to quantify ZEA and its reduced metabolites in subcellular fractions of animal livers (
Guerre, Philippe +3 more
core +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Virulence, 2018 Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia
Jéssica Andrade Paes +6 more
doaj +1 more source
Journal of Lipid Research, 1966 The roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-14C (0.005%) supplemented with cholestanol-7α-3H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ...
N.L. Lasser, R.B. Clayton
doaj +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Binding of radioactive methylmercuric chloride in subcellular fractions of neuroblastoma and glioma cells in culture [PDF]
, 1979 The binding of radioactive methylmercuric chloride ([203Hg] -CH3HgCl) with various subcellular fractions of glioma (C-6) and neuroblastoma (NBP2) cells was studied. Confluent cells were incubated in the presence of radioactive CH3HgCl for a period of 5 h,
Prasad, KN, Ramanujam, M, Bondy, SC
core +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source