Results 11 to 20 of about 95,299 (308)

Preparation of Crude Subcellular Fractions by Differential Centrifugation [PDF]

open access: yesThe Scientific World Journal, 2002
The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
doaj   +2 more sources

Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups

open access: yesActa Biomedica Scientifica, 2013
With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human ...
S. N. Kozlov   +4 more
doaj   +2 more sources

Ligand-Specific Regulation of the Endogenous Mu-Opioid Receptor by Chronic Treatment with Mu-Opioid Peptide Agonist [PDF]

open access: yes, 2013
Since the discovery of the endomorphins (EM), the postulated endogenous peptide agonists of the mu-opioid receptors, several analogues have been synthesized to improve their binding and pharmacological profiles.
Murányi Marianna   +25 more
core   +1 more source

Status epilepticus-induced changes in the subcellular distribution and activity of calcineurin in rat forebrain

open access: yesNeurobiology of Disease, 2003
This study was performed to determine the effect of prolonged status epilepticus on the activity and subcellular location of a neuronally enriched, calcium-regulated enzyme, calcineurin. Brain fractions isolated from control animals and rats subjected to
Jonathan E Kurz   +3 more
doaj   +1 more source

A New Targeted Lipidomics Approach Reveals Lipid Droplets in Liver, Muscle and Heart as a Repository for Diacylglycerol and Ceramide Species in Non-Alcoholic Fatty Liver

open access: yesCells, 2019
Obesity is frequently associated with excessive accumulation of lipids in ectopic tissue and presents a major risk factor for type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD). Diacylglycerols (DAGs) and ceramides (CERs) were identified
Christina Preuss   +7 more
doaj   +1 more source

Cholesterol Depletion Alters Cardiomyocyte Subcellular Signaling and Increases Contractility. [PDF]

open access: yesPLoS ONE, 2016
Membrane cholesterol levels play an important factor in regulating cell function. Sarcolemmal cholesterol is concentrated in lipid rafts and caveolae, which are flask-shaped invaginations of the plasma membrane.
Mohammed Z Haque   +4 more
doaj   +1 more source

Spatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver, and Lung Metastasis

open access: yesCells, 2022
Metastasis is the primary cause of colorectal cancer (CRC) death. The liver and lung, besides adjacent lymph nodes, are the most common sites of metastasis.
Guillermo Solís-Fernández   +13 more
doaj   +1 more source

Subcellular Fractionation of Cultured Human Cell Lines

open access: yesBio-Protocol, 2013
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer.
Zhuoyou Yu, Zhiguang Huang, Maria Lung
doaj   +1 more source

Cholesterol metabolism in myelin and other subcellular fractions of rat brain

open access: yesJournal of Lipid Research, 1972
For many years the bulk of myelin in adult brain was believed to be metabolically stable, although some metabolic activity of a small myelin fraction, especially in the gray matter of the brain, was recognized.
Martha Spohn, A.N. Davison
doaj   +1 more source

Subcellular localization of hexadecanedioic acid activation in human liver

open access: yesJournal of Lipid Research, 1974
The activation of hexadecanedioic acid has been studied in subcellular fractions of human liver. The activation capacity in a total homogenate of human liver was found to be 0.5 μmole/min/g wet wt of tissue, about 10% of that for palmitic acid ...
Jon Elling Pettersen, Magne Aas
doaj   +1 more source

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