Results 1 to 10 of about 326,046 (306)

Cell Fractionation and the Identification of Host Proteins Involved in Plant–Virus Interactions [PDF]

Pathogens
Plant viruses depend on host cellular factors for their replication and movement. There are cellular proteins that change their localization and/or expression and have a proviral role or antiviral activity and interact with or target viral proteins ...
Amany E. Gomaa, Kaoutar El Mounadi, Eric Parperides, Hernan Garcia-Ruiz   +3 more
doaj   +2 more sources

Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions [PDF]

BIO-PROTOCOL, 2021
Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them.
Ankita Arora, Raeann Goering, Hei-Yong Lo, J. Taliaferro   +3 more
openaire   +3 more sources

The Existence and Localization of Nuclear snoRNAs in Arabidopsis thaliana Revisited

Plants, 2020
Ribosome biogenesis is one cell function-defining process. It depends on efficient transcription of rDNAs in the nucleolus as well as on the cytosolic synthesis of ribosomal proteins. For newly transcribed rRNA modification and ribosomal protein assembly,
Deniz Streit, Thiruvenkadam Shanmugam, Asen Garbelyanski, Stefan Simm, Enrico Schleiff   +4 more
doaj   +1 more source

Simple and Efficient Protocol for Subcellular Fractionation of Normal and Apoptotic Cells

Cells, 2021
Subcellular fractionation approaches remain an indispensable tool among a large number of biochemical methods to facilitate the study of specific intracellular events and characterization of protein functions.
Viacheslav V. Senichkin, Evgeniia A. Prokhorova, Boris Zhivotovsky, Gelina S. Kopeina   +3 more
doaj   +1 more source

Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis [PDF]

, 2009
Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain.
AJ Driessen, BJ Feilmeier, C Filip, CA Kumamoto, CF Schierle, CM Cho, CR Raetz, D Huber, D Schmid, D Tullman-Ercek, DP Heuts, E Brickman, E Frirdich, E Vrontou, EW van Hellemond, F Forneris, FJ Mergulhao, HC Jung, J Jose, J Luirink, J Zhou, JD Thomas, JS Lee, K Nakai, L Li, M Marrichi, M Shimazu, M Shimazu, MA Turner, MJ Casadaban, MJ Casadaban, ML Wu, MW Fraaije, NG Leferink, P Samuelson, PA Lee, PH Wu, R Federico, S Cristobal, SK Yim, SM Kang, SP Graether, SY Lee, T Baba, T Bruser   +44 more
core   +9 more sources

A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts

STAR Protocols, 2021
Summary: This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability.
Gabriella E. Farrugia, Micheal A. McLellan, Kate L. Weeks, Aya Matsumoto, Charles D. Cohen, Crisdion Krstevski, Taylah L. Gaynor, Adam C. Parslow, Julie R. McMullen, Alexander R. Pinto   +9 more
doaj   +1 more source

Functional Features of Alginates Recovered from Himanthalia elongata Using Subcritical Water Extraction

Molecules, 2021
Subcritical water extraction of Himanthalia elongata and the subsequent acetone fractionation to precipitate crude fucoidans generated a liquid phase which was used to recover alginates with a wide range of viscoelastic features and other soluble ...
Noelia Flórez-Fernández, Herminia Domínguez, María Dolores Torres   +2 more
doaj   +1 more source

Intracellular events in the assembly of chylomicrons in rabbit enterocytes

Journal of Lipid Research, 2000
The aim of this study was to determine the intracellular events in chylomicron assembly in adult villus enterocytes. We have used novel methods for separation of the intracellular components of the secretory compartment [rough and smooth endoplasmic ...
Ian J. Cartwright, Dietmar Plonné, Joan A. Higgins   +2 more
doaj   +1 more source

Optimizing radiation therapy treatments by exploring tumour ecosystem dynamics in-silico [PDF]

, 2019
In this contribution, we propose a system-level compartmental population dynamics model of tumour cells that interact with the patient (innate) immune system under the impact of radiation therapy (RT).
Fellermann, Harold, Scheidegger, Stephan
core   +1 more source

Isolation of autophagic fractions from mouse liver for biochemical analyses

STAR Protocols, 2021
Summary: Isolation of autophagosomes, autolysosomes, and lysosomes allows mechanistic studies into the pathophysiology of autophagy—a lysosomal quality control pathway.
Henrietta Bains, Rajat Singh
doaj   +1 more source