Results 21 to 30 of about 180,687 (266)

Subcellular Fractionation of Cultured Human Cell Lines

Bio-Protocol, 2013
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer.
Zhuoyou Yu, Zhiguang Huang, Maria Lung
doaj   +1 more source

REAP: A two minute cell fractionation method

BMC Research Notes, 2010
Background The translocation or shuttling of proteins between the nucleus and cytoplasm (nucleocytoplasmic transport [NCPT]) is often a rapid event following stimulation with growth factors or in response to stress or other experimental manipulations ...
Fujita Donald J, Leong-Quong Rebecca YY, Bose Pinaki, Suzuki Keiko, Riabowol Karl   +4 more
doaj   +1 more source

Investigations on Zinc Isotope Fractionation in Breast Cancer Tissue Using in vitro Cell Culture Uptake-Efflux Experiments

Frontiers in Medicine, 2022
Zinc (Zn) accumulates in breast cancer tumors compared to adjacent healthy tissue. Clinical samples of breast cancer tissue show light Zn isotopic compositions (δ66Zn) relative to healthy tissue. The underlying mechanisms causing such effects are unknown.
Kathrin Schilling, Kathrin Schilling, Kathrin Schilling, Adrian L. Harris, Alex N. Halliday, Alex N. Halliday, Christopher J. Schofield, Helen Sheldon, Syed Haider, Fiona Larner, Fiona Larner   +10 more
doaj   +1 more source

Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy

STAR Protocols, 2023
Summary: Here, we present a protocol to observe the three-dimensional surface of nuclear pore complexes (NPCs) of vertebrate early embryos by field emission scanning electron microscopy (FESEM).
Weimin Shen, Anming Meng
doaj   +1 more source

NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells

Bio-Protocol, 2017
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells.
Alvaro E. Galvis, Hugh Fisher, David Camerini   +2 more
doaj   +1 more source

Fractionation of Hepatic Nonparenchymal Cells [PDF]

The Scientific World JOURNAL, 2002
The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells.
openaire   +3 more sources

A method for the analysis of the oligomerization profile of the Huntington’s disease-associated, aggregation-prone mutant huntingtin protein by isopycnic ultracentrifugation

Frontiers in Molecular Biosciences
Conformational diseases, such as Alzheimer’s, Parkinson’s and Huntington’s diseases as well as ataxias and fronto-temporal disorders, are part of common class of neurological disorders characterised by the aggregation and progressive accumulation of ...
Raffaella Bonavita, Raffaella Bonavita, Rosaria Di Martino, Rosaria Di Martino, Giuseppe Cortone, Antonello Prodomo, Mariagrazia Di Gennaro, Mariagrazia Di Gennaro, Mariagrazia Di Gennaro, Gianluca Scerra, Valentino Panico, Silvia Nuzzo, Marco Salvatore, Sarah V. Williams, Fulvia Vitale, Maria Gabriella Caporaso, Massimo D’Agostino, Francesca M. Pisani, Angeleen Fleming, Maurizio Renna, Maurizio Renna   +20 more
doaj   +1 more source

ALTERED FRACTIONATION RADIOTHERAPY FOR HEAD AND NECK CANCER

Žurnal Grodnenskogo Gosudarstvennogo Medicinskogo Universiteta, 2019
The purpose of the review is to analyze and summarize literature data about the role of altered fractionation radiotherapy for head and neck cancer. A total of 46 English-language literature sources were analyzed. They provide a radiobiological rationale
Parkhomenko L. B.
doaj   +1 more source

Genetic Cell-Surface Modification for Optimized Foam Fractionation

Frontiers in Bioengineering and Biotechnology, 2020
Rhamnolipids are among the glycolipids that have been investigated intensively in the last decades, mostly produced by the facultative pathogen Pseudomonas aeruginosa using plant oils as carbon source and antifoam agent.
Christian C. Blesken, Isabel Bator, Isabel Bator, Christian Eberlein, Hermann J. Heipieper, Till Tiso, Till Tiso, Lars M. Blank, Lars M. Blank   +8 more
doaj   +1 more source

Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

STAR Protocols, 2022
Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such
Cai Han, Linyu Sun, Qi Pan, Yumeng Sun, Wentao Wang, Yueqin Chen   +5 more
doaj   +1 more source