Results 31 to 40 of about 326,046 (306)

Subcellular Fractionation of Cultured Human Cell Lines

open access: yesBio-Protocol, 2013
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer.
Zhuoyou Yu, Zhiguang Huang, Maria Lung
doaj   +1 more source

Protocol to determine the subcellular localization of protein interactions in murine keratinocytes

open access: yesSTAR Protocols, 2023
Summary: Protein activities and interactions are determined by their subcellular localization. Elucidating the network of protein-protein interactions at a spatial resolution is essential for understanding the complexity of protein functions, their ...
Lisa Müller
doaj   +1 more source

Association of p60c-src with endosomal membranes in mammalian fibroblasts. [PDF]

open access: yes, 1992
We have examined the subcellular localization of p60c-src in mammalian fibroblasts. Analysis of indirect immunofluorescence by three-dimensional optical sectioning microscopy revealed a granular cytoplasmic staining that co-localized with the microtubule
Kaplan, KB   +3 more
core   +1 more source

REAP: A two minute cell fractionation method

open access: yesBMC Research Notes, 2010
Background The translocation or shuttling of proteins between the nucleus and cytoplasm (nucleocytoplasmic transport [NCPT]) is often a rapid event following stimulation with growth factors or in response to stress or other experimental manipulations ...
Fujita Donald J   +4 more
doaj   +1 more source

NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells

open access: yesBio-Protocol, 2017
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells.
Alvaro E. Galvis   +2 more
doaj   +1 more source

Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

open access: yesSTAR Protocols, 2022
Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such
Cai Han   +5 more
doaj   +1 more source

Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]

open access: yes, 2012
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D.   +11 more
core   +2 more sources

ALTERED FRACTIONATION RADIOTHERAPY FOR HEAD AND NECK CANCER

open access: yesŽurnal Grodnenskogo Gosudarstvennogo Medicinskogo Universiteta, 2019
The purpose of the review is to analyze and summarize literature data about the role of altered fractionation radiotherapy for head and neck cancer. A total of 46 English-language literature sources were analyzed. They provide a radiobiological rationale
Parkhomenko L. B.
doaj   +1 more source

Petrology of some oceanic island basalts: PRIMELT2.XLS software for primary magma calculation [PDF]

open access: yes, 2008
PRIMELT2.XLS software is introduced for calculating primary magma composition and mantle potential temperature (TP) from an observed lava composition. It is an upgrade over a previous version in that it includes garnet peridotite melting and it detects ...
Asimow, Paul D., Herzberg, C.
core   +2 more sources

A method for the analysis of the oligomerization profile of the Huntington’s disease-associated, aggregation-prone mutant huntingtin protein by isopycnic ultracentrifugation

open access: yesFrontiers in Molecular Biosciences
Conformational diseases, such as Alzheimer’s, Parkinson’s and Huntington’s diseases as well as ataxias and fronto-temporal disorders, are part of common class of neurological disorders characterised by the aggregation and progressive accumulation of ...
Raffaella Bonavita   +20 more
doaj   +1 more source

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