Results 31 to 40 of about 326,046 (306)
Subcellular Fractionation of Cultured Human Cell Lines
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer.
Zhuoyou Yu, Zhiguang Huang, Maria Lung
doaj +1 more source
Protocol to determine the subcellular localization of protein interactions in murine keratinocytes
Summary: Protein activities and interactions are determined by their subcellular localization. Elucidating the network of protein-protein interactions at a spatial resolution is essential for understanding the complexity of protein functions, their ...
Lisa Müller
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Association of p60c-src with endosomal membranes in mammalian fibroblasts. [PDF]
We have examined the subcellular localization of p60c-src in mammalian fibroblasts. Analysis of indirect immunofluorescence by three-dimensional optical sectioning microscopy revealed a granular cytoplasmic staining that co-localized with the microtubule
Kaplan, KB +3 more
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REAP: A two minute cell fractionation method
Background The translocation or shuttling of proteins between the nucleus and cytoplasm (nucleocytoplasmic transport [NCPT]) is often a rapid event following stimulation with growth factors or in response to stress or other experimental manipulations ...
Fujita Donald J +4 more
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NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells.
Alvaro E. Galvis +2 more
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Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such
Cai Han +5 more
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Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D. +11 more
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ALTERED FRACTIONATION RADIOTHERAPY FOR HEAD AND NECK CANCER
The purpose of the review is to analyze and summarize literature data about the role of altered fractionation radiotherapy for head and neck cancer. A total of 46 English-language literature sources were analyzed. They provide a radiobiological rationale
Parkhomenko L. B.
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Petrology of some oceanic island basalts: PRIMELT2.XLS software for primary magma calculation [PDF]
PRIMELT2.XLS software is introduced for calculating primary magma composition and mantle potential temperature (TP) from an observed lava composition. It is an upgrade over a previous version in that it includes garnet peridotite melting and it detects ...
Asimow, Paul D., Herzberg, C.
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Conformational diseases, such as Alzheimer’s, Parkinson’s and Huntington’s diseases as well as ataxias and fronto-temporal disorders, are part of common class of neurological disorders characterised by the aggregation and progressive accumulation of ...
Raffaella Bonavita +20 more
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