Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligera [PDF]
Frontiers in Cell and Developmental Biology, 2023 Background:Polycarpa mytiligera is the only molecularly characterized solitary ascidian capable of regenerating all organs and tissue types. The cellular basis for regeneration in P.
Tal Gordon +3 more
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Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties
Micromachines, 2017 In molecular and cellular biological research, cell isolation and sorting are required for accurate investigation of a specific cell types. By employing unique cell properties to distinguish between cell types, rapid and accurate sorting with high ...
Caffiyar Mohamed Yousuff +3 more
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Protocol for optimized mononuclear cell isolation from liver and tumor tissue using mechanical or enzymatic digestion [PDF]
STAR ProtocolsSummary: Mononuclear cells from human liver tissue, including tumor and non-tumor regions, are a valuable source for studies of the tumor microenvironment. Here, we present a protocol to dissociate liver tissue from patients with hepatocellular carcinoma.
Sachin Kumar Singh Chauhan +4 more
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Corrigendum – Targeting Toll-Like Receptor 4: a promising strategy to prevent type 1 diabetes occurrence or recurrence [PDF]
CellR4, 2022 Corrigendum
M. Alibashe-Ahmed +3 more
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Targeting Toll-Like Receptor 4: a promising strategy to prevent type 1 diabetes occurrence or recurrence [PDF]
CellR4, 2020 TLR4 is a transmembrane receptor of the innate immune system that recognize LPS of gram-negative bacteria. Its stimulation induces pro-inflammatory responses and also modulates adaptive immunity.
M. Alibashe-Ahmed +3 more
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Not reimbursing islet transplantation creates discrimination against patients with type 1 diabetes [PDF]
CellR4, 2019 In this commentary reviewing the activity of the University of Geneva islet transplantation program, the author argues that health care coverage of the procedure will stop discrimination against a subset of patients with type 1 diabetes in need of beta ...
T. Berney
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Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
STAR Protocols, 2021 Summary: Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue ...
Courtney J. Phifer +7 more
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Cell isolation and culture [PDF]
WormBook, 2013 Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui, Zhang, Jeffrey R, Kuhn
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STUDIES ON ISOLATED CELL COMPONENTS [PDF]
Journal of General Physiology, 1951 1. The addition of heparin to rat liver, kidney, or brain nuclei has been found to bring about the release of a gel. Chemical analysis and histochemical studies on whole homogenates and isolated nuclei demonstrated that the material released by heparin contained desoxyribonucleic acid (DNA) and protein. The action of heparin on nuclei is interpreted as
N G, ANDERSON, K M, WILBUR
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Frontiers in Bioengineering and Biotechnology, 2022 Vascularization of tissue-engineered constructs remains a key challenge in the field of skeletal muscle tissue engineering. One strategy for vascularizing organoids is in vitro pre-vascularization, relying on de novo assembly of undifferentiated ...
Rebecca Wüst +4 more
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