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Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligera [PDF]

open access: yesFrontiers in Cell and Developmental Biology, 2023
Background:Polycarpa mytiligera is the only molecularly characterized solitary ascidian capable of regenerating all organs and tissue types. The cellular basis for regeneration in P.
Tal Gordon   +3 more
doaj   +2 more sources

Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties

open access: yesMicromachines, 2017
In molecular and cellular biological research, cell isolation and sorting are required for accurate investigation of a specific cell types. By employing unique cell properties to distinguish between cell types, rapid and accurate sorting with high ...
Caffiyar Mohamed Yousuff   +3 more
doaj   +3 more sources

Protocol for optimized mononuclear cell isolation from liver and tumor tissue using mechanical or enzymatic digestion [PDF]

open access: yesSTAR Protocols
Summary: Mononuclear cells from human liver tissue, including tumor and non-tumor regions, are a valuable source for studies of the tumor microenvironment. Here, we present a protocol to dissociate liver tissue from patients with hepatocellular carcinoma.
Sachin Kumar Singh Chauhan   +4 more
doaj   +2 more sources

Targeting Toll-Like Receptor 4: a promising strategy to prevent type 1 diabetes occurrence or recurrence [PDF]

open access: yesCellR4, 2020
TLR4 is a transmembrane receptor of the innate immune system that recognize LPS of gram-negative bacteria. Its stimulation induces pro-inflammatory responses and also modulates adaptive immunity.
M. Alibashe-Ahmed   +3 more
doaj   +1 more source

Not reimbursing islet transplantation creates discrimination against patients with type 1 diabetes [PDF]

open access: yesCellR4, 2019
In this commentary reviewing the activity of the University of Geneva islet transplantation program, the author argues that health care coverage of the procedure will stop discrimination against a subset of patients with type 1 diabetes in need of beta ...
T. Berney
doaj   +1 more source

Obtaining patient-derived cancer organoid cultures via fine-needle aspiration

open access: yesSTAR Protocols, 2021
Summary: Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue ...
Courtney J. Phifer   +7 more
doaj   +1 more source

Efficient co-isolation of microvascular endothelial cells and satellite cell-derived myoblasts from human skeletal muscle

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Vascularization of tissue-engineered constructs remains a key challenge in the field of skeletal muscle tissue engineering. One strategy for vascularizing organoids is in vitro pre-vascularization, relying on de novo assembly of undifferentiated ...
Rebecca Wüst   +4 more
doaj   +1 more source

Author Correction: Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

open access: yesNature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Fanny Lebreton   +13 more
doaj   +1 more source

Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

open access: yesFrontiers in Immunology, 2021
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz   +4 more
doaj   +1 more source

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