Results 41 to 50 of about 1,131,780 (265)
Isolation of Tissue Mast Cells
Current Protocols in Immunology, 2010 AbstractLocated primarily in tissues, mast cells are one of the principal effector cells in allergic inflammation. Mast cells derive from mononuclear precursor cells which undergo their final phase of differentiation in the tissues. Mast cells express a unique set of proteases and display functional diversity depending on the tissue in which they ...
Kulka, Marianna, Metcalfe, Dean D.
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Single Cell Isolation and Analysis [PDF]
Frontiers in Cell and Developmental Biology, 2016 Individual cell heterogeneity within a population can be critical to its peculiar function and fate. Subpopulations studies with mixed mutants and wild types may not be as informative regarding which cell responds to which drugs or clinical treatments. Cell to cell differences in RNA transcripts and protein expression can be key to answering questions ...
Hu, Ping +3 more
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The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia
Pediatric Blood &Cancer, EarlyView.ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli +3 more
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Numerical Modeling of Physical Cell Trapping in Microfluidic Chips
Micromachines, 2023 Microfluidic methods have proven to be effective in separation and isolation of cells for a wide range of biomedical applications. Among these methods, physical trapping is a label-free isolation approach that relies on cell size as the selective ...
Sara Cardona +5 more
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FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
STAR ProtocolsSummary: 3D culture models better replicate tumor structure and interactions than 2D methods, enhancing cancer therapy research. Here, we present a protocol to develop a 3D mouse autologous lung tumor-immune spheroid co-culture model to advance ...
Sergio León +8 more
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Dissecting phenotypic transitions in metastatic disease via photoconversion-based isolation
eLife, 2021 Cancer patients often harbor occult metastases, a potential source of relapse that is targetable only through systemic therapy. Studies of this occult fraction have been limited by a lack of tools with which to isolate discrete cells on spatial grounds ...
Yogev Sela +7 more
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Ischemic Preconditioning in Isolated Cells [PDF]
Circulation Research, 2000 Ischemic preconditioning describes the increased resistance to myocardial infarction that follows short sublethal periods of ischemia. After the ischemia that triggers preconditioning, there are 2 phases of protection: an early (short-lived) phase termed early or classic preconditioning1 and a late (more prolonged) phase termed late preconditioning or ...
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Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
STAR ProtocolsSummary: Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. We describe steps for combining anatomical dissection of the mandibular alveolar region with flow cytometry analysis ...
Kouta Niizuma +6 more
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