Results 21 to 30 of about 1,131,780 (265)
Bioengineering, 2023 Mesenchymal stem cells (MSCs) hold considerable promise for regenerative medicine. Optimization of the seeding density of mononuclear cells (MNCs) improves the proliferative and differentiation potential of isolated MSCs.
Hiroyuki Nagai +4 more
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Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes
Nature Communications, 2019 Islet transplantation is a feasible approach to treat type I diabetes, however inflammation and poor vascularisation impair long-term engraftment. Here the authors show that incorporating human amniotic epithelial cells into islet organoids improves ...
Fanny Lebreton +13 more
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Measures of viability in isolated cells [PDF]
Biochemical Medicine, 1975 Abstract Various methods were compared for estimation of cell viability. Cell count, uptake of trypan blue, release of cellular LDH, as well as cell contents of K+ and Na+ were determined in leukocytes, spleen cells, and Ehrlich cells exposed to adverse conditions. After incubation of these cells with NaF, the cell count remained essentially constant;
Medzihradsky, Fedor, Marks, Michael J.
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Isolation of Circulating Melanoma Cells [PDF]
, 2015 Circulating melanoma cells (CMCs) represent critical mediators of metastatic melanoma progression. However, isolation and characterization of CMCs has been challenging due to the low frequency of these cells and the paucity of melanoma-specific cell surface markers.
Jie, Ma, Markus H, Frank
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Cell Transplantation, 2006 Early revascularization of pancreatic islet cells after transplantation is crucial for engraftment, and it has been suggested that vascular endothelial growth factor-A (VEGF-A) plays a significant role in this process.
Zoltan Mathe +8 more
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Purification of retinal ganglion cells using low-pressure flow cytometry
Frontiers in Molecular Neuroscience, 2023 Purified Retinal Ganglion Cells (RGCs) for in vitro study have been a valuable tool in the study of neural regeneration and in the development of therapies to treat glaucoma.
Kiran J. Mcloughlin +11 more
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Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation
Cell Transplantation, 2017 Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers.
Daniel Brandhorst +10 more
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Isolation of Microvascular Endothelial Cells
BIO-PROTOCOL, 2018 The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol demonstrates a quick, efficient method for the isolation of microvascular endothelial cells from murine tissues ...
Kenneth Cheung, Federica Marelli-Berg
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Isolation of circulating tumor cells
iScience, 2022 Circulating tumor cells (CTCs) enter the vasculature from solid tumors and disseminate widely to initiate metastases. Mining the metastatic-enriched molecular signatures of CTCs before, during, and after treatment holds unique potential in personalized oncology.
Jon F. Edd +6 more
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Cell Transplantation, 2010 Ex vivo labeling of islets with superparamagnetic iron oxide (SPIO) nanoparticles allows posttransplant MRI imaging of the graft. In the present study, we compare two clinical grade SPIOs (ferucarbotran and ferumoxide) in terms of toxicity, islet ...
Frederic Ris M.D. +12 more
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