Results 21 to 30 of about 6,713,855 (257)

iMEM: Isolation of Plasma Membrane for Cryoelectron Microscopy. [PDF]

, 2016
The plasma membrane and the cell cortex are essential parts of the eukaryotic cell. The plasma membrane delimitates the cell and mediates communication with the outside.
Beckmann, Sven, Zuber, Benoît, Peitsch, Camille   +2 more
core   +1 more source

Tetracycline-Regulated Expression of VEGF-A in Beta Cells Induces Angiogenesis: Improvement of Engraftment following Transplantation

Cell Transplantation, 2006
Early revascularization of pancreatic islet cells after transplantation is crucial for engraftment, and it has been suggested that vascular endothelial growth factor-A (VEGF-A) plays a significant role in this process.
Zoltan Mathe, Philippe Dupraz, Chris Rinsch, Bernard Thorens, Domenico Bosco, Marie Zbinden, Philippe Morel, Thierry Berney, Michael S. Pepper   +8 more
doaj   +1 more source

Purification of retinal ganglion cells using low-pressure flow cytometry

Frontiers in Molecular Neuroscience, 2023
Purified Retinal Ganglion Cells (RGCs) for in vitro study have been a valuable tool in the study of neural regeneration and in the development of therapies to treat glaucoma.
Kiran J. Mcloughlin, Kiran J. Mcloughlin, Afnan M. Aladdad, Afnan M. Aladdad, Andrew J. Payne, Andrew J. Payne, Anna I. Boda, Anna I. Boda, Sayra Nieto-Gomez, Sayra Nieto-Gomez, Karl E. Kador, Karl E. Kador   +11 more
doaj   +1 more source

Two homologous Agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1 [PDF]

, 2008
A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and ...
Vos, W.M., de, Kleerebezem, M., Kunuki, Ryoko, Ingham, C.J., Sturme, Mark, Nakayama, J., Vaughan, Elaine, Kunuki, R., Nakayama, Jiro, Sturme, M.H.J., Fujii, Toshio, Kleerebezem, Michiel, Molenaar, D., Fujii, T., Vaughan, E.E., Ingham, Colin, Beerthuyzen, Marke, Molenaar, Douwe, de Vos, Willem, Molenaar, Douwe; id_orcid, de Vos, W.M., Beerthuyzen, M.M.   +21 more
core   +1 more source

Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation

Cell Transplantation, 2017
Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers.
Daniel Brandhorst, Géraldine Parnaud, Andrew Friberg, Vanessa Lavallard, Sandrine Demuylder-Mischler, Stephen Hughes, Julia Saphörster, Manfred Kurfürst, Olle Korsgren, Thierry Berney, Paul R. V. Johnson   +10 more
doaj   +1 more source

Waste Isolation Pilot Plant Environmental Monitoring Plan [PDF]

, 1999
DOE Order 5400.1, General Environmental Protection Program Requirements (DOE, 1990a), requires each DOE facility to prepare an EMP. This document is prepared for WIPP in accordance with the guidance contained in DOE Order 5400.1; DOE Order 5400.5 ...
Division, Westinghouse Electric Company Waste Isolation
core   +1 more source

Assessment of Human Islet Labeling with Clinical Grade Iron Nanoparticles Prior to Transplantation for Graft Monitoring by MRI

Cell Transplantation, 2010
Ex vivo labeling of islets with superparamagnetic iron oxide (SPIO) nanoparticles allows posttransplant MRI imaging of the graft. In the present study, we compare two clinical grade SPIOs (ferucarbotran and ferumoxide) in terms of toxicity, islet ...
Frederic Ris M.D., Matthieu Lepetit-Coiffe, Paolo Meda, Lindsey A. Crowe, Christian Toso, Mathieu Armanet, Nadja Niclauss, Géraldine Parnaud, Laurianne Giovannoni, Domenico Bosco, Philippe Morel, Jean-Paul Vallee, Thierry Berney   +12 more
doaj   +1 more source

Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing [PDF]

, 2021
Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies.
Schneider, Rebekka K., Leimkühler, Nils B., Fuchs, Stijn N. R., Snoeren, Inge A. M., Fuchs, Stijn N.R., Gleitz, Hélène F.E., Gleitz, Hélène F. E., Snoeren, Inge A.M.   +7 more
core   +1 more source

VyCAP's puncher technology for single cell identification, isolation, and analysis [PDF]

, 2018
Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes.
Weersink, Joris, Swennenhuis, Joost Franciscus, Tibbe, Arjan G.J., Broekmaat, Joska Johannes, Oomens, Lisa, Broekmaat, Joska J., Stevens, Michiel, Abali, Fekri, Swennenhuis, Joost F.   +8 more
core   +2 more sources

Experimental Considerations for Single-Cell RNA Sequencing Approaches

Frontiers in Cell and Developmental Biology, 2018
Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell biology is largely limited to data generated by bulk profiling methods ...
Quy H. Nguyen, Nicholas Pervolarakis, Kevin Nee, Kai Kessenbrock   +3 more
doaj   +1 more source