Results 11 to 20 of about 6,713,855 (257)

Microfluidic cancer cell isolation system using magnetic nanoparticle hybrids and advanced interdigitated electrode technology [PDF]

, 2010
For the past decade in the biomedical engineering industry, extensive research has been conducted in improving magnetic sensing technologies for cell isolation systems. These technologies, through the popular biomarkers, i.e.
Mahendru, Dishant
core   +6 more sources

Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation [PDF]

Iranian Journal of Immunology, 2023
Background: An issue that hinders researchers’ access to Natural Killer (NK) cells is their low proportion in peripheral blood leukocytes. This issue is currently addressed by methods involving a series of differentiation and expansions that are time ...
Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah   +3 more
doaj   +1 more source

Proposed practical protocol for flow cytometry analysis of microglia from the healthy adult mouse brain: Systematic review and isolation methods’ evaluation

Frontiers in Cellular Neuroscience, 2022
The aim of our study was to systematically analyze the literature for published flow cytometry protocols for microglia isolation and compare their effectiveness in terms of microglial yield, including our own protocol using sucrose for myelin removal and
Sanja Srakočić, Paula Josić, Sebastijan Trifunović, Srećko Gajović, Danka Grčević, Anton Glasnović   +5 more
doaj   +1 more source

High-dimensional analysis of intestinal immune cells during helminth infection

eLife, 2020
Single cell isolation from helminth-infected murine intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration.
Laura Ferrer-Font, Palak Mehta, Phoebe Harmos, Alfonso J Schmidt, Sally Chappell, Kylie M Price, Ian F Hermans, Franca Ronchese, Graham le Gros, Johannes U Mayer   +9 more
doaj   +1 more source

The Endplate Role in Degenerative Disc Disease Research: The Isolation of Human Chondrocytes from Vertebral Endplate—An Optimised Protocol

Bioengineering, 2022
Background: Degenerative disc disease is a progressive and chronic disorder with many open questions regarding its pathomorphological mechanisms. In related studies, in vitro organ culture systems are becoming increasingly essential as a replacement ...
Lidija Gradišnik, Uroš Maver, Boris Gole, Gorazd Bunc, Matjaž Voršič, Janez Ravnik, Tomaž Šmigoc, Roman Bošnjak, Tomaž Velnar   +8 more
doaj   +1 more source

An optimized protocol for coupling oxygen consumption rates with β-oxidation in isolated mitochondria from mouse soleus

STAR Protocols, 2021
Summary: Depending on metabolic requirements, skeletal muscle mitochondria integrate O2 consumption and ATP production with lipid, glucose, or amino acid metabolism.
Cristina Sánchez-González, Laura Formentini   +1 more
doaj   +1 more source

Burn-injured skin is marked by a prolonged local acute inflammatory response of innate immune cells and pro-inflammatory cytokines

Frontiers in Immunology, 2022
The systemic and local immune response in burn patients is often extreme and derailed. As excessive inflammation can damage healthy tissues and slow down the healing process, modulation of inflammatory responses could limit complications and improve ...
Patrick P.G. Mulder, Patrick P.G. Mulder, Marcel Vlig, Esther Fasse, Matthea M. Stoop, Anouk Pijpe, Anouk Pijpe, Anouk Pijpe, Anouk Pijpe, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Irma Joosten, Bouke K.H.L. Boekema, Bouke K.H.L. Boekema, Hans J.P.M. Koenen   +16 more
doaj   +1 more source

Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes. [PDF]

Adv Sci (Weinh)
Wang C, Qiu J, Liu M, Wang Y, Yu Y, Liu H, Zhang Y, Han L.   +7 more
europepmc   +2 more sources

Optimizing the Seeding Density of Human Mononuclear Cells to Improve the Purity of Highly Proliferative Mesenchymal Stem Cells

Bioengineering, 2023
Mesenchymal stem cells (MSCs) hold considerable promise for regenerative medicine. Optimization of the seeding density of mononuclear cells (MNCs) improves the proliferative and differentiation potential of isolated MSCs.
Hiroyuki Nagai, Akihiro Miwa, Kenji Yoneda, Koichi Fujisawa, Taro Takami   +4 more
doaj   +1 more source

Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

Nature Communications, 2019
Islet transplantation is a feasible approach to treat type I diabetes, however inflammation and poor vascularisation impair long-term engraftment. Here the authors show that incorporating human amniotic epithelial cells into islet organoids improves ...
Fanny Lebreton, Vanessa Lavallard, Kevin Bellofatto, Romain Bonnet, Charles H. Wassmer, Lisa Perez, Vakhtang Kalandadze, Antonia Follenzi, Michel Boulvain, Julie Kerr-Conte, David J. Goodman, Domenico Bosco, Thierry Berney, Ekaterine Berishvili   +13 more
doaj   +1 more source