Results 11 to 20 of about 1,131,780 (265)

Author Correction: Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Fanny Lebreton, Vanessa Lavallard, Kevin Bellofatto, Romain Bonnet, Charles H. Wassmer, Lisa Perez, Vakhtang Kalandadze, Antonia Follenzi, Michel Boulvain, Julie Kerr-Conte, David J. Goodman, Domenico Bosco, Thierry Berney, Ekaterine Berishvili   +13 more
doaj   +1 more source

Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation [PDF]

Iranian Journal of Immunology, 2023
Background: An issue that hinders researchers’ access to Natural Killer (NK) cells is their low proportion in peripheral blood leukocytes. This issue is currently addressed by methods involving a series of differentiation and expansions that are time ...
Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah   +3 more
doaj   +1 more source

Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

Frontiers in Immunology, 2021
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz, Manuel Obermaier, Sandra Uhle, Markus Alexander Weigand, Florian Uhle   +4 more
doaj   +1 more source

Technologies for Single-Cell Isolation [PDF]

International Journal of Molecular Sciences, 2015
The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis.
Gross, André, Schoendube, Jonas, Zimmermann, Stefan, Steeb, Maximilian, Zengerle, Roland, Koltay, Peter   +5 more
openaire   +3 more sources

Microfluidic Biochips for Single‐Cell Isolation and Single‐Cell Analysis of Multiomics and Exosomes [PDF]

Advanced Science
Lin Han, Hong Liu, Yihe Wang
exaly   +2 more sources

Proposed practical protocol for flow cytometry analysis of microglia from the healthy adult mouse brain: Systematic review and isolation methods’ evaluation

Frontiers in Cellular Neuroscience, 2022
The aim of our study was to systematically analyze the literature for published flow cytometry protocols for microglia isolation and compare their effectiveness in terms of microglial yield, including our own protocol using sucrose for myelin removal and
Sanja Srakočić, Paula Josić, Sebastijan Trifunović, Srećko Gajović, Danka Grčević, Anton Glasnović   +5 more
doaj   +1 more source

High-dimensional analysis of intestinal immune cells during helminth infection

eLife, 2020
Single cell isolation from helminth-infected murine intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration.
Laura Ferrer-Font, Palak Mehta, Phoebe Harmos, Alfonso J Schmidt, Sally Chappell, Kylie M Price, Ian F Hermans, Franca Ronchese, Graham le Gros, Johannes U Mayer   +9 more
doaj   +1 more source

The Endplate Role in Degenerative Disc Disease Research: The Isolation of Human Chondrocytes from Vertebral Endplate—An Optimised Protocol

Bioengineering, 2022
Background: Degenerative disc disease is a progressive and chronic disorder with many open questions regarding its pathomorphological mechanisms. In related studies, in vitro organ culture systems are becoming increasingly essential as a replacement ...
Lidija Gradišnik, Uroš Maver, Boris Gole, Gorazd Bunc, Matjaž Voršič, Janez Ravnik, Tomaž Šmigoc, Roman Bošnjak, Tomaž Velnar   +8 more
doaj   +1 more source

An optimized protocol for coupling oxygen consumption rates with β-oxidation in isolated mitochondria from mouse soleus

STAR Protocols, 2021
Summary: Depending on metabolic requirements, skeletal muscle mitochondria integrate O2 consumption and ATP production with lipid, glucose, or amino acid metabolism.
Cristina Sánchez-González, Laura Formentini   +1 more
doaj   +1 more source

Burn-injured skin is marked by a prolonged local acute inflammatory response of innate immune cells and pro-inflammatory cytokines

Frontiers in Immunology, 2022
The systemic and local immune response in burn patients is often extreme and derailed. As excessive inflammation can damage healthy tissues and slow down the healing process, modulation of inflammatory responses could limit complications and improve ...
Patrick P.G. Mulder, Patrick P.G. Mulder, Marcel Vlig, Esther Fasse, Matthea M. Stoop, Anouk Pijpe, Anouk Pijpe, Anouk Pijpe, Anouk Pijpe, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Paul P.M. van Zuijlen, Irma Joosten, Bouke K.H.L. Boekema, Bouke K.H.L. Boekema, Hans J.P.M. Koenen   +16 more
doaj   +1 more source