Results 51 to 60 of about 6,713,855 (257)
STAR ProtocolsSummary: Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. We describe steps for combining anatomical dissection of the mandibular alveolar region with flow cytometry analysis ...
Kouta Niizuma +6 more
doaj +1 more source
Dissecting phenotypic transitions in metastatic disease via photoconversion-based isolation
eLife, 2021 Cancer patients often harbor occult metastases, a potential source of relapse that is targetable only through systemic therapy. Studies of this occult fraction have been limited by a lack of tools with which to isolate discrete cells on spatial grounds ...
Yogev Sela +7 more
doaj +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Nerve cell differentiation in hydra requires two signals [PDF]
, 1986 Endogenous signals controlling nerve cell commitment in hydra were investigated using an assay for committed nerve precursors. Extracts of hydra tissue were prepared and tested for their ability to induce nerve cell commitment.
Schaller, Chica H. +2 more
core +1 more source
Islets-on-Chip: A Tool for Real-Time Assessment of Islet Function Prior to Transplantation
Transplant International, 2023 Islet transplantation improves metabolic control in patients with unstable type 1 diabetes. Clinical outcomes have been improving over the last decade, and the widely used beta-score allows the evaluation of transplantation results.
Matthieu Raoux +11 more
doaj +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Waste Isolation Pilot Plant 1999 Site Environmental Report [PDF]
, 2000 The U.S. Department of Energy s (DOE)Carlsbad Area Office and the Westinghouse Waste Isolation Division (WID) are dedicated to maintaining high quality management of Waste Isolation Pilot Plant (WIPP) environmental resources.
Division, Westinghouse Waste Isolation +6 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
FEBS Letters, EarlyView.Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure +3 more
wiley +1 more source
Establishment of pluripotent cell lines from vertebrate species - Present status and future prospects [PDF]
, 1999 Pluripotent embryonic stem (ES) cells are undifferentiated cell lines derived from early embryos and are capable of unlimited undifferentiated proliferation in vitro.
Vassilieva, Svetlana G. +4 more
core +1 more source