Results 61 to 70 of about 6,713,855 (257)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

open access: yesFEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part II: Gene regulation [PDF]

open access: yes, 2012
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study ...
Julianna Kobolak   +17 more
core   +1 more source

Dual-well microfluidic technique for single cell isolation and long-term clonal culture

open access: yes, 2023
[[abstract]]The clonal culture of single cells is widely used for studying cell dynamics and heterogeneity in vitro and producing monoclonal cell populations.
Yeh, CF;Chang, HC;Hsu, CH
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Alternative cell line for the isolation of salmonid alphavirus-1 [PDF]

open access: yes, 2009
Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon ...
Adams, Alexandra   +4 more
core  

Liver-derived extracellular vesicles: A cell by cell overview to isolation and characterization practices. [PDF]

open access: yes, 2020
BACKGROUND: Extracellular vesicles (EVs) are a diverse group of membrane-bound nanovesicles potentially released by every cell. With the liver's unique ensemble of cells and its fundamental physiological tasks, elucidating the role of EV-mediated hepatic
Luciani, Paola   +2 more
core   +3 more sources

Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

open access: yesSTAR Protocols
Summary: The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of pro- to anti-inflammatory phenotypes.
Pierre Lemaitre   +2 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

Dorsal Raphe Dopamine Neurons Represent the Experience of Social Isolation

open access: yes, 2015
The motivation to seek social contact may arise from either positive or negative emotional states, as social interaction can be rewarding and social isolation can be aversive.
Halbert, Sarah A.   +12 more
core   +1 more source

Mesenchymal cell invasion requires cooperative regulation of persistent microtubule growth by SLAIN2 and CLASP1 [PDF]

open access: yes, 2016
Microtubules regulate signaling, trafficking, and cell mechanics, but the respective contribution of these functions to cell morphogenesis and migration in 3D matrices is unclear.
Noordstra, Ivar   +15 more
core   +2 more sources

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