Results 41 to 50 of about 2,285 (215)
Crude subcellular fractionation of cultured mammalian cell lines
BMC Research Notes, 2009 Background The expression and study of recombinant proteins in mammalian culture systems can be complicated during the cell lysis procedure by contaminating proteins from cellular compartments distinct from those within which the protein of interest ...
Holden Paul, Horton William A
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Protocol to establish a stable MLL-AF9_AML mouse model
STAR Protocols, 2022 Summary: Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse MLL-AF9_AML model to investigate AML.
Jun Lu +3 more
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FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
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Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins
STAR Protocols, 2023 Summary: Here, we provide a protocol to isolate mitochondria from cultured cells and extract differently located mitochondrial proteins. We detail steps to separate both integral and peripheral membrane proteins from soluble proteins using sonication. We
Danyi Zhou +5 more
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FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Cell fractionation of parasitic protozoa: a review
Memorias do Instituto Oswaldo Cruz, 2003 Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of investigators.
Souza Wanderley de +1 more
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Single‐Cell Nucleus Extraction with Cellular Indexing
Advanced ScienceBulk organelle‑fractionation masks cell‑to‑cell heterogeneity, and existing microfluidic methods cannot reliably reconnect each isolated organelle to its parent cell, an essential capability for multiomics readouts.
Trinh Lam +7 more
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Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Tecnología para la utilización integral de la levadura de cerveza en la industria alimenticia
Archivos Latinoamericanos de Nutrición, 2000 Se desarrolló un esquema flexible para el fraccionamiento de la levadura de cerveza. El procedimiento permite la producción de diferentes productos tales como: hojuelas de levadura, tabletas de levadura, salsa de mesa a base de extracto de levadura ...
Miguel A. Otero +4 more
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