Results 11 to 20 of about 180,687 (266)
Cell Fractionation and the Identification of Host Proteins Involved in Plant–Virus Interactions [PDF]
PathogensPlant viruses depend on host cellular factors for their replication and movement. There are cellular proteins that change their localization and/or expression and have a proviral role or antiviral activity and interact with or target viral proteins ...
Amany E. Gomaa +3 more
doaj +2 more sources
Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions [PDF]
BIO-PROTOCOL, 2021 Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them.
Ankita Arora +3 more
openaire +3 more sources
The Existence and Localization of Nuclear snoRNAs in Arabidopsis thaliana Revisited
Plants, 2020 Ribosome biogenesis is one cell function-defining process. It depends on efficient transcription of rDNAs in the nucleolus as well as on the cytosolic synthesis of ribosomal proteins. For newly transcribed rRNA modification and ribosomal protein assembly,
Deniz Streit +4 more
doaj +1 more source
Simple and Efficient Protocol for Subcellular Fractionation of Normal and Apoptotic Cells
Cells, 2021 Subcellular fractionation approaches remain an indispensable tool among a large number of biochemical methods to facilitate the study of specific intracellular events and characterization of protein functions.
Viacheslav V. Senichkin +3 more
doaj +1 more source
A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts
STAR Protocols, 2021 Summary: This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability.
Gabriella E. Farrugia +9 more
doaj +1 more source
Molecules, 2021 Subcritical water extraction of Himanthalia elongata and the subsequent acetone fractionation to precipitate crude fucoidans generated a liquid phase which was used to recover alginates with a wide range of viscoelastic features and other soluble ...
Noelia Flórez-Fernández +2 more
doaj +1 more source
Intracellular events in the assembly of chylomicrons in rabbit enterocytes
Journal of Lipid Research, 2000 The aim of this study was to determine the intracellular events in chylomicron assembly in adult villus enterocytes. We have used novel methods for separation of the intracellular components of the secretory compartment [rough and smooth endoplasmic ...
Ian J. Cartwright +2 more
doaj +1 more source
Isolation of autophagic fractions from mouse liver for biochemical analyses
STAR Protocols, 2021 Summary: Isolation of autophagosomes, autolysosomes, and lysosomes allows mechanistic studies into the pathophysiology of autophagy—a lysosomal quality control pathway.
Henrietta Bains, Rajat Singh
doaj +1 more source
Fractionation of the insoluble material of Chlorella cells [PDF]
Biochimica et Biophysica Acta, 1960 Abstract A procedure is described for the fractionation of Chlorella cell material, labeled with 14C, based on solvent extraction followed by serial digestion with several enzymes, and extraction of the breakdown products following each enzymic treatment.
Stange, Luis, Moses, V., Calvin, M.
openaire +4 more sources
Frontiers in Microbiology, 2017 We investigated the influence of organic substrates and phosphate concentration on the rates of dissimilatory microbial sulfate reduction and the 34S/32S isotopic fractionation produced by several Desulfovibrio species.
Shikma Zaarur +3 more
doaj +1 more source