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Specific Fractionation of Immunocompetent Cells
Immunological Reviews, 1970Prominent progress in molecular biology was only made when it became possible to separate functionally distinct molecules by taking advantage of their biophysical properties. Likewise, the analysis of the functions of heter ogeneous populations of immunocompetent cells, as to the functional properties of their various subpopulations, can not be done ...
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1976
Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Differential Detergent Fractionation of Eukaryotic Cells
Cold Spring Harbor Protocols, 2005INTRODUCTIONDifferential detergent fractionation (DDF) involves the sequential extraction of cells with PIPES buffers containing first digitonin, then Triton, and finally Tween/deoxycholate (DOC). The procedure yields four biochemically and electrophoretically distinct fractions composed of the following: (1) cytosolic proteins and extractable ...
Melinda, Ramsby, Gregory, Makowski
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Lipolytic activity in adipocyte cell fractions
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Adipocytes release only negligible amounts of free fatty acids unless stimulated, but reveal considerable lipolytic activity when homogenized. Epinephrine treatment of the cells caused only a 20-40% increase in the activity of infranatants of homogenates while raising the activity associated with the fat layer up to 10-fold. Full activity (i.e. that of
Y, Oschry, B, Shapiro
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Cell Disruption and Fractionation
2011The living cell contains a number of subcellular fractions. The fractionation of cells involves two distinct phases: disruption of the tissue or cells in a suitable medium and the subsequent separation of the subcellular particles, by differential centrifugation which exploits differences in their size and density. The procedure results in rather crude
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Electron Microscopy: Cytology of Cell Fractions
Science, 1956It should be evident from this brief account that electron microscopy of thin sections is an invaluable asset in the study of fractions isolated by differential centrifugation. I have tried to indicate how the integrity of particles, purity of fractions, and the existence of new particles can be established through its use.
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Cell fractionation biochemistryand the origins of ‘cell biology’
Trends in Biochemical Sciences, 1996openaire +2 more sources