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, 2014 Proteins within a cell are localized into specific cellular compartments, allowing compartmentalization of distinct tasks. If we consider lipid bilayers as compartments, then gram-negative bacteria such as Pseudomonas aeruginosa can target proteins to five distinct locations: the cytoplasm, the inner membrane, the periplasm, the outer membrane, and the
Ize, Bérengère +2 more
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Continuous split flow-thin cell fractionation of starch particles
Journal of Chromatography A, 1999 The fractionation of starch granules was investigated using a SPLITT (split flow-thin) cell, a relatively new system for fast, continuous binary separations.
Catia Contado
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Overview of Cell Fractionation
Current Protocols in Protein Science, 1995AbstractThis discussion unit describes the most common methods for cell fractionation which provides the essential ingredients for the increasing number of cell‐free assays now being used in test‐tube reconstructions of complex cellular events involving intercompartmental interactions.
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BIOCHEMISTRY OF CELL FRACTIONS
Canadian Journal of Biochemistry and Physiology, 1957Null
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Biochemical Fractionation of Drosophila Cells
2007This chapter describes how to perform basic biochemical fractionations of Drosophila cells, and how to begin to characterize the proteins in the resulting fractions. The protocols include maintenance and transfection of Drosophila cell lines (Section 3.1.), hypotonic lysis (Section 3.2.), and separation of cellular lysates into cytosolic and membrane ...
Melanie, Stegman, David, Robbins
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Cellular Fractionation – Yeast Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Cell Fractionation of Parasitic Protozoa
2008Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of researchers. These studies have provided new information of the cell biology of these parasites and have supported investigators to assume that some of the protozoa form the roots of the ...
Wanderley, de Souza +2 more
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Cellular Fractionation – Mammalian Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Trends in Biochemical Sciences, 1980
Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Yair Reisner, Nathan Sharon
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Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Yair Reisner, Nathan Sharon
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