Results 221 to 230 of about 180,687 (266)
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Differential Detergent Fractionation of Eukaryotic Cells
Cold Spring Harbor Protocols, 2005INTRODUCTIONDifferential detergent fractionation (DDF) involves the sequential extraction of cells with PIPES buffers containing first digitonin, then Triton, and finally Tween/deoxycholate (DOC). The procedure yields four biochemically and electrophoretically distinct fractions composed of the following: (1) cytosolic proteins and extractable ...
Melinda, Ramsby, Gregory, Makowski
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1976
Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Interactions of hormones with thymus-cell fractions
Biochimica et Biophysica Acta (BBA) - General Subjects, 1964Abstract The binding of cortisol, progesterone and testosterone to thymus-cell fractions was studied by the equilibrium dialysis procedure. The hormones appear to follow the “polarity rule”, progesterone binding to the greatest extent, cortisol the least.
W K, BRUNKHORST, E L, HESS
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Subcellular fractionation of tissue culture cells
Trends in Biochemical Sciences, 1989Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the ...
K E, Howell, E, Devaney, J, Gruenberg
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Cell Disruption and Fractionation
2011The living cell contains a number of subcellular fractions. The fractionation of cells involves two distinct phases: disruption of the tissue or cells in a suitable medium and the subsequent separation of the subcellular particles, by differential centrifugation which exploits differences in their size and density. The procedure results in rather crude
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Electron Microscopy: Cytology of Cell Fractions
Science, 1956It should be evident from this brief account that electron microscopy of thin sections is an invaluable asset in the study of fractions isolated by differential centrifugation. I have tried to indicate how the integrity of particles, purity of fractions, and the existence of new particles can be established through its use.
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Fractionation of cells and subcellular particles with Percoll
Journal of Biochemical and Biophysical Methods, 2000At present, centrifugation is the most common method for separation and isolation of cells and subcellular particles. The technique can be used for a wide range of applications. During latter years it has become obvious what a powerful method density gradient centrifugation is, especially when used in conjunction with sensitive assays or clinical ...
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The lignin fraction of plant cell walls
The American Journal of Clinical Nutrition, 1978Methods are discussed for determining lignin in plant cell walls. The increase in apparent lignin content that may occur as a result of artifacts produced during food preparation is also discussed. The phenolic components, including lignin, of cell walls separated from 12 vegetable, fruit, and cereal foods are determined.
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