Results 71 to 80 of about 12,578,619 (347)

Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation

STAR Protocols
Summary: Here, we present a protocol for the direct isolation of small extracellular vesicles (sEVs) from the spleen of preclinical murine models of leukemia using ultracentrifugation.
Ernesto Gargiulo, Pablo Elias Morande, Maxmilan Jeyakumar, Lucie Rospape, Jérôme Paggetti, Etienne Moussay   +5 more
doaj   +1 more source

Export of miRNAs from activated macrophages is cooperative and HuR‐dependent

FEBS Letters, EarlyView.
This study uncovers a new mechanism for miRNA export regulation in mammalian cells, driven by the RNA‐binding protein HuR. In macrophages, HuR cooperatively binds with miRNAs to export both low‐ and high‐affinity substrates, thereby influencing macrophage activation.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Protocol for the isolation and purification of endoplasmic reticulum-plasma membrane junctions from the mouse brain

STAR Protocols
Summary: Dynamic communication between intracellular organelles often takes place at specialized membrane contact sites that form between their membranes.
Jason A. Weesner, Diantha van de Vlekkert, Leigh Ellen Fremuth, Alessandra d’Azzo   +3 more
doaj   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

The Combination of Temporal and Spatial Dose Fractionation in Microbeam Radiation Therapy

Biomedicines
Background: Microbeam radiation therapy (MRT) is an advanced preclinical approach in radiotherapy that utilizes spatially fractionated dose distributions by collimating x-rays into micrometer-wide, planar beams.
Jessica Stolz, Kristina Rogal, Sandra Bicher, Johanna Winter, Mabroor Ahmed, Susanne Raulefs, Stephanie E. Combs, Stefan H. Bartzsch, Thomas E. Schmid   +8 more
doaj   +1 more source

Tumor and germline testing with next generation sequencing in epithelial ovarian cancer: a prospective paired comparison using an 18‐gene panel

Molecular Oncology, EarlyView.
Genetic testing in epithelial ovarian cancer includes both germline and tumor‐testing. This approach often duplicates resources. The current prospective study assessed the feasibility of tumor‐first multigene testing by comparing tumor tissue with germline testing of peripheral blood using an 18‐gene NGS panel in 106 patients.
Elisabeth Spenard, Cristina Mitric, Melanie Care, Tracy L. Stockley, Raymond H. Kim, Jeanna McCuaig, Blaise Clarke, Laura Ranich, Clare Sheen, Sarah E. Ferguson, Liat Hogen, Taymaa May, Marcus Q. Bernardini   +12 more
wiley   +1 more source

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome

Nano Convergence, 2018
In this article, we elaborate the application of thermal decomposition based synthesis of Fe3O4 superparamagnetic nanoparticle (SPMNP) in subcellular fractionation context.
Deepak B. Thimiri Govinda Raj, Niamat Ali Khan   +1 more
doaj   +1 more source

Genotoxic Bystander Signals from Irradiated Human Mesenchymal Stromal Cells Mainly Localize in the 10–100 kDa Fraction of Conditioned Medium [PDF]

, 2021
Vanessa Kohl, Alice Fabarius, Oliver Drews, Miriam Bierbaum, Ahmed Jawhar, Ali Darwich, Christel Weiß, Johanna Flach, Susanne Brendel, Helga Kleiner, Wolfgang Seifarth, Wolf‐Karsten Hofmann, Henning D. Popp   +12 more
openalex   +1 more source