Results 61 to 70 of about 12,492,811 (359)

Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics

Micromachines, 2021
This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a ...
Honeyeh Matbaechi Ettehad, Christian Wenger   +1 more
doaj   +1 more source

Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae*

Journal of Biological Chemistry, 2016
The human-restricted pathogen Neisseria gonorrhoeae encodes a single N-acetylmuramyl-l-alanine amidase involved in cell separation (AmiC), as compared with three largely redundant cell separation amidases found in Escherichia coli (AmiA, AmiB, and AmiC).
J. Lenz, Elizabeth A. Stohl, Rosanna M. Robertson, K. Hackett, K. Fisher, K. Xiong, Mijoon Lee, D. Hesek, S. Mobashery, H. Steven Seifert, C. Davies, J. P. Dillard   +11 more
semanticscholar   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Biomedical Applications of Polymeric Cryogels

Applied Sciences, 2019
The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over
Monireh Bakhshpour, Neslihan Idil, Işık Perçin, Adil Denizli   +3 more
doaj   +1 more source

Fast Mechanically Driven Daughter Cell Separation Is Widespread in Actinobacteria

mBio, 2016
Dividing cells of the coccoid Gram-positive bacterium Staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (DCS) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically ...
Xiaoxue Zhou, David K. Halladin, J. Theriot   +2 more
semanticscholar   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Modelling and prototyping of a microfluidic cartridge for extracting immune cells from small volume blood samples

Current Directions in Biomedical Engineering, 2020
Within the human blood immune cells assume specific functions to modulate and control the human immune response. Consequently, they are used for the diagnosis and treatment of autoimmune and hematologic diseases.
Behrens Stephan, Sonntag Frank, Schmieder Florian   +2 more
doaj   +1 more source

Magnetic cell separation

, 2022
Magnetic cell separation, also termed magnetic-activated cell sorting, refers to the separation technology that employs magnetic fields to differentiate various cell populations and isolates target cells from the biological sample. Due to the superior advantages such as low cost, simple operation, high selectivity, high throughput, robustness, and good
Zhang, J, Nguyen, NT
openaire   +2 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source