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Chapter 9.6 Cell Surface Antigens
1983Publisher Summary This chapter highlights research obtained with one species of cell surface macromolecules—that is, human histocompatibility antigens that polyacrylamide gel electrophoresis (PAGE) and immunoprecipitates–sodium dodecyl sulphate–polyacrylamide gel electrophoresis (IP-SDS-PAGE) made.
R.A. Reisfeld, M.A. Pellegrino
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Hapten — Sandwich Labeling of Cell-Surface Antigens
1978The indirect antibody technique for amplified detection of antigens (Coons, 1956) has a wide and expanding range of applications. In general, this well-established method employs second-layer antiimmunoglobulin, coupled to an appropriate detector, to label first-layer antibody specific for a particular antigen.
L, Wofsy, C, Henry, S, Cammisuli
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Surface Antigens of Murine Natural Killer Cells
1988Publisher Summary This chapter provides an overview of the current status of the surface antigens and markers that are specific for natural killer (NK) cells and others that are not confined to NK cells. As murine NK cells are heterogeneous, the chapter discusses the NK cells recovered from murine spleen that exhibit lytic activity toward lymphoma ...
R C, Burton +4 more
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The Cell-surface Antigens of Bacteroides vulgatus
Microbiology, 1981The cell surface of Bacteroides vulgatus was examined by electron microscopy. The outer membrane complex was removed by EDTA and mild sonication and the antigens of this complex were characterized by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis.
I R, Poxton, M K, Ip
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Monoclonal Antibodies to Mouse Cell‐Surface Antigens
Current Protocols in Immunology, 2002AbstractThe cluster of differentiation (CD) nomenclature, originally devised by the International Workshop on Human Leukocyte Differentiation Antigens (HLDA) to classify leukocyte surface antigens, has become more accepted by researchers in nonhuman animal models.
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Differentiation Antigens of the Lymphocyte Cell Surface
Biochemical Society Transactions, 1976Many alloantigens and xenoantigens of lymphocytes are not found generally on other tissues, and this suggests that much of the lymphocyte cell surface is differentiated in comparison with other cell membranes. These differentiation antigens are probably molecules that mediate lymphocyte-specific functions, and are also of interest in that they provide ...
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Silver nanoparticles for the detection of cell surface antigens in mass cytometry
Cytometry Part A, 2017A. Schulz +4 more
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Monoclonal antibodies defining distinctive human T cell surface antigens.
Science, 1979P. Kung +3 more
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Enzymatic Amplification Staining for Cell Surface Antigens
Current Protocols in Cytometry, 2003AbstractMany molecules of biological significance function at very low levels and are difficult to detect with the standard staining procedures for flow cytometric analysis. This unit describes a technology for cell staining that enhances the specific fluorescence signal by as much as 100‐fold.
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