Results 31 to 40 of about 802,703 (319)

Molecular architecture of the PBP2–MreC core bacterial cell wall synthesis complex

open access: yesNature Communications, 2017
Bacterial wall biosynthesis is a complex process that requires the coordination of multiple enzymes. Here, the authors structurally characterize the PBP2:MreC complex involved in peptidoglycan elongation and cross-linking, and demonstrate that its ...
Carlos Contreras-Martel   +9 more
doaj   +1 more source

Substituted Cysteine Accessibility Method for Topology and Activity Studies of Membrane Enzymes Forming Thioester Acyl Intermediates in Bacteria

open access: yesBio-Protocol, 2015
The topology of membrane proteins and enzymes can be determined using various methods including reporter protein fusions and accessibility of cysteine residues to alkylating agents.
Sébastien Gélis-Jeanvoine   +1 more
doaj   +1 more source

Cell Wall Biology: Perspectives from Cell Wall Imaging [PDF]

open access: yesMolecular Plant, 2011
Polysaccharide-rich plant cell walls are important biomaterials that underpin plant growth, are major repositories for photosynthetically accumulated carbon, and, in addition, impact greatly on the human use of plants. Land plant cell walls contain in the region of a dozen major polysaccharide structures that are mostly encompassed by cellulose ...
J. Paul Knox   +2 more
openaire   +3 more sources

A case of Beauveria bassiana keratitis confirmed by internal transcribed spacer and LSU rDNA D1–D2 sequencing

open access: yesNew Microbes and New Infections, EarlyView., 2014
Abstract We describe a case of fungal keratitis due to Beauveria bassiana in a farmer with Fuchs' dystrophy, treated with amphotericin B. Surgery with penetrating keratoplasty was necessary to resolve the lesions. Susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive ...
M. Ligozzi   +8 more
wiley   +1 more source

Creep Characterization of Inconel 718 Lattice Metamaterials Manufactured by Laser Powder Bed Fusion

open access: yesAdvanced Engineering Materials, EarlyView., 2023
Herein, the creep characteristics of additively manufactured Inconel 718 metamaterials are investigated. The creep behavior of metamaterials and the effects of microstructural defects are assessed, and the microstructure defects are accurately captured using Kachanov's creep damage model.
Akash Singh Bhuwal   +5 more
wiley   +1 more source

Characterization of the cell wall and cell wall proteins of Chromatium vinosum [PDF]

open access: yesJournal of Bacteriology, 1980
Highly purified cell walls of Chromatium vinosum were isolated by differential centrifugation, with or without Triton X-100 extraction. The isolated material had a protein composition similar to that of cell walls obtained by sucrose density gradient centrifugation. Twenty-two proteins were reproducibly detected by sodium dodecyl sulfate-polyacrylamide
B C Lane, R E Hurlbert
openaire   +2 more sources

Conceptual Design and Parametric Optimization of a New Multileveled Horsetail Structure for Bicycle Helmets

open access: yesAdvanced Engineering Materials, EarlyView., 2023
This study aims to explore the feasibility of using a structure inspired by the features of horsetail and human spine as the potential helmet liner, targeting at mitigation of acceleration‐induced injuries. A parametric study is conducted to investigate the effect of individual geometrical variables in the design, indicating its capability to reduce ...
Bing Leng   +3 more
wiley   +1 more source

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls [PDF]

open access: yesPlant Methods 2 (2006) 10, 2006
The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess specific difficulties. Several reported procedures to isolate cell walls for proteomic analyses led to the isolation of a high proportion (more than 50%) of
arxiv   +1 more source

Deconstructing the Cell Wall [PDF]

open access: yesPlant Physiology, 1994
Any microorganism that attempts to colonize a plant must contend with the cell wall. One of the most conspicuous effects of microorganisms on plant cell walls is enzymic degradation. Our interpretation of the significance of this depends on our concept of the plant cell wall, which, however, is paradoxical.
openaire   +3 more sources

Topology Optimization of Lattice Support Structure for Cantilever Beams Fabricated Via Laser Powder Bed Fusion

open access: yesAdvanced Engineering Materials, EarlyView., 2023
A numerical scheme is presented to design a lattice support for metallic components additively built via laser powder bed fusion. Results show that thermal‐induced distortion can be respectively reduced by 69%, 58%, and 50% in comparison to a uniform lattice, a fully solid support, and a truss‐based lattice support.
Jiazheng Hu   +2 more
wiley   +1 more source

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