Results 101 to 110 of about 942,792 (355)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Unraveling the mechanism of TTL genes in cellulose biosynthesis [PDF]

, 2019
As sessile organisms, plants require mechanisms to sense and respond to the challenging environment, that encompass both biotic and abiotic factors that results in differential development.
Amorim-Silva, Vitor, Botella-Mesa, Miguel Angel, Castillo-Garriga, Araceli, García Moreno, Álvaro, Jaillais, Yvon, Kesten, Christopher, Macho, Alberto, Menna, Alexandra, Sanchez-Rodriguez, Clara, Valpuesta-Fernandez, Victoriano   +9 more
core  

Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass [PDF]

, 2018
Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic biomass a suitable feedstock for the bio-based economy, plants can be engineered to have decreased amounts of lignin.
Boerjan, Wout, Corneillie, Sander, De Bruyne, Michiel, De Meester, Barbara, De Rycke, Riet, de Vries, Lisanne, Gierlinger, Notburga, Goeminne, Geert, Morreel, Kris, Pallidis, Andreas, Vanholme, Ruben, Özparpucu, Merve   +11 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Cell Wall Integrity MAPK Pathway Is Essential for Lipid Homeostasis [PDF]

Journal of Biological Chemistry, 2008
The highly conserved yeast cell wall integrity mitogen-activated protein kinase pathway regulates cellular responses to cell wall and membrane stress. We report that this pathway is activated and essential for viability under growth conditions that alter both the abundance and pattern of synthesis and turnover of membrane phospholipids, particularly ...
Lilia R, Nunez, Stephen A, Jesch, Maria L, Gaspar, Claudia, Almaguer, Manuel, Villa-Garcia, Monica, Ruiz-Noriega, Jana, Patton-Vogt, Susan A, Henry   +7 more
openaire   +2 more sources

The basic leucine zipper transcription factor MeaB is critical for biofilm formation, cell wall integrity, and virulence in Aspergillus fumigatus

mSphere
The regulation of fungal cell wall biosynthesis is crucial for cell wall integrity maintenance and directly impacts fungal pathogen virulence. Although numerous genes are involved in fungal cell wall polysaccharide biosynthesis through multiple pathways,
Yuan Chen, Fei Gao, Xiaojin Chen, Siyuan Tao, Peiying Chen, Wei Lin   +5 more
doaj   +1 more source

Atkinesin-13A modulates cell-wall synthesis and cell expansion in Arabidopsis thaliana via the THESEUS1 pathway. [PDF]

PLoS Genetics, 2014
Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited.
Ushio Fujikura, Lore Elsaesser, Holger Breuninger, Clara Sánchez-Rodríguez, Alexander Ivakov, Thomas Laux, Kim Findlay, Staffan Persson, Michael Lenhard   +8 more
doaj   +1 more source

The Role of the Cell Integrity Pathway in Septum Assembly in Yeast

Journal of Fungi, 2021
Cytokinesis divides a mother cell into two daughter cells at the end of each cell cycle and proceeds via the assembly and constriction of a contractile actomyosin ring (CAR).
Cesar Roncero, Rubén Celador, Noelia Sánchez, Patricia García, Yolanda Sánchez   +4 more
doaj   +1 more source

Titan cell production in Cryptococcus neoformans reshapes the cell wall and capsule composition during infection [PDF]

, 2018
This work was supported by the National Institutes of Health (R01AI080275 and R21AI22352), the NIH Fogarty International Center (R25TW009345), the University of Minnesota Center for Translational Science Institute (UL1TR000114), Wellcome Trust (086827 ...
Gow, Neil A R, Lee, Keunsook, Mukaremera, Liliane, Nielsen, Kirsten, Wagener, Jeanette, Wiesner, D. L.   +5 more
core   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source