Characterization of the cell wall and cell wall proteins of Chromatium vinosum [PDF]
Highly purified cell walls of Chromatium vinosum were isolated by differential centrifugation, with or without Triton X-100 extraction. The isolated material had a protein composition similar to that of cell walls obtained by sucrose density gradient centrifugation. Twenty-two proteins were reproducibly detected by sodium dodecyl sulfate-polyacrylamide
B C, Lane, R E, Hurlbert
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The regulation of the cell wall by glycosylphosphatidylinositol-anchored proteins in Arabidopsis
A polysaccharides-based cell wall covers the plant cell, shaping it and protecting it from the harsh environment. Cellulose microfibrils constitute the cell wall backbone and are embedded in a matrix of pectic and hemicellulosic polysaccharides and ...
Ke Zhou
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The Neurospora crassa dfg5 and dcw1 genes encode α-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall. [PDF]
The covalent cross-linking of cell wall proteins into the cell wall glucan/chitin matrix is an important step in the biogenesis of the fungal cell wall.
Abhiram Maddi, Ci Fu, Stephen J Free
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Yeast cell wall stability is important for cell division and survival under stress conditions. The expression of cell-wall-related proteins is regulated by several pathways involving RNA-binding proteins and RNases.
Ana Novačić +4 more
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Cell wall proteins of Aquaspirillum serpens [PDF]
The Triton X-100-insoluble wall fraction of Aquaspirillum serpens VHA contained three major proteins: the regularly structured (RS) superficial protein (molecular weight 140,000) and two peptidoglycan-associated proteins (molecular weights, 32,000 and 33,000).
S F, Koval, R G, Murray
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Dynamic protein trafficking to the cell wall [PDF]
Recently we have studied the secretion pattern of a pectin methylesterase inhibitor protein (PMEI1) and a polygalacturonase inhibitor protein (PGIP2) in tobacco protoplast using the protein fusions, secGFP-PMEI1 and PGIP2-GFP. Both chimeras reach the cell wall by passing through the endomembrane system but using distinct mechanisms and through a ...
DE CAROLI, MONICA +5 more
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Isolation of Plant Cell Wall Proteins [PDF]
The quality of a proteomic analysis of a cell compartment strongly depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific drawbacks: (1) the lack of a surrounding membrane may result in the loss of cell wall proteins (CWP) during the isolation procedure; (2) polysaccharide networks ...
Jamet, Elisabeth +7 more
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Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall ofSaccharomyces cerevisiae [PDF]
Tagging of two cell wall mannoproteins, Cwp1p and Cwp2p, in Saccharomyces cerevisiae with the green fluorescent protein from Aequorea victoria resulted in incorporation of fluorescent fusion proteins into the cell wall. Both living cells and isolated cell walls became brightly labeled.
Ram, A.F.J. +2 more
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New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress. [PDF]
BACKGROUND AND AIMS: Copper (Cu) is an essential micronutrient for plants. However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
Tingting Liu +4 more
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Proteomic analysis of cell walls of two developmental stages of alfalfa stems
Cell walls are important for the growth and development of all plants. They are also valuable resources for feed and fiber, and more recently as a potential feedstock for bioenergy production. Cell wall proteins comprise only a fraction of the cell wall,
Julian C Verdonk +2 more
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