Genome-wide screen identifies novel factors for surface protein cross-wall trafficking and cell envelope homeostasis in Staphylococcus aureus [PDF]
Cell-wall-anchored surface proteins are integral components of gram-positive bacterial cell envelopes. The trafficking of many surface proteins carrying a YSIRK/G-S signal peptide is synchronized with cell envelope biogenesis during cell division ...
Salvatore J. Scaffidi +3 more
doaj +2 more sources
Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins
Cell walls play key roles during plant development. Following their deposition into the cell wall, polysaccharides are continually remodeled according to the growth stage and stress environment to accommodate cell growth and differentiation. To date, little is known concerning the enzymes involved in cell wall remodeling, especially in gramineous and ...
Francin-Allami, Mathilde +9 more
openaire +4 more sources
Characterization of the cell wall and cell wall proteins of Chromatium vinosum [PDF]
Highly purified cell walls of Chromatium vinosum were isolated by differential centrifugation, with or without Triton X-100 extraction. The isolated material had a protein composition similar to that of cell walls obtained by sucrose density gradient centrifugation. Twenty-two proteins were reproducibly detected by sodium dodecyl sulfate-polyacrylamide
B C, Lane, R E, Hurlbert
openaire +2 more sources
The regulation of the cell wall by glycosylphosphatidylinositol-anchored proteins in Arabidopsis
A polysaccharides-based cell wall covers the plant cell, shaping it and protecting it from the harsh environment. Cellulose microfibrils constitute the cell wall backbone and are embedded in a matrix of pectic and hemicellulosic polysaccharides and ...
Ke Zhou
doaj +1 more source
The Neurospora crassa dfg5 and dcw1 genes encode α-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall. [PDF]
The covalent cross-linking of cell wall proteins into the cell wall glucan/chitin matrix is an important step in the biogenesis of the fungal cell wall.
Abhiram Maddi, Ci Fu, Stephen J Free
doaj +1 more source
Yeast cell wall stability is important for cell division and survival under stress conditions. The expression of cell-wall-related proteins is regulated by several pathways involving RNA-binding proteins and RNases.
Ana Novačić +4 more
doaj +1 more source
Cell wall proteins of Aquaspirillum serpens [PDF]
The Triton X-100-insoluble wall fraction of Aquaspirillum serpens VHA contained three major proteins: the regularly structured (RS) superficial protein (molecular weight 140,000) and two peptidoglycan-associated proteins (molecular weights, 32,000 and 33,000).
S F, Koval, R G, Murray
openaire +2 more sources
Dynamic protein trafficking to the cell wall [PDF]
Recently we have studied the secretion pattern of a pectin methylesterase inhibitor protein (PMEI1) and a polygalacturonase inhibitor protein (PGIP2) in tobacco protoplast using the protein fusions, secGFP-PMEI1 and PGIP2-GFP. Both chimeras reach the cell wall by passing through the endomembrane system but using distinct mechanisms and through a ...
DE CAROLI, MONICA +5 more
openaire +3 more sources
Isolation of Plant Cell Wall Proteins [PDF]
The quality of a proteomic analysis of a cell compartment strongly depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific drawbacks: (1) the lack of a surrounding membrane may result in the loss of cell wall proteins (CWP) during the isolation procedure; (2) polysaccharide networks ...
Jamet, Elisabeth +7 more
openaire +3 more sources
Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall ofSaccharomyces cerevisiae [PDF]
Tagging of two cell wall mannoproteins, Cwp1p and Cwp2p, in Saccharomyces cerevisiae with the green fluorescent protein from Aequorea victoria resulted in incorporation of fluorescent fusion proteins into the cell wall. Both living cells and isolated cell walls became brightly labeled.
Ram, A.F.J. +2 more
openaire +4 more sources

