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Homologous Expression of Recombinant Cellobiose Dehydrogenase in Phanerochaete chrysosporium
Biochemical and Biophysical Research Communications, 2000Cellobiose dehydrogenase (CDH) is a novel extracellular hemoflavoenzyme from Phanerochaete chrysosporium and is produced only in cultures supplemented with cellulose. In this report, CDH from P. chrysosporium has been homologously expressed in cultures supplemented with glucose as the sole carbon source when no endogenous CDH is expressed.
B, Li +3 more
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Production and characterization of cellobiose dehydrogenase
2016The fungal enzyme cellobiose dehydrogenase (CDH) is a monomeric oxidoreductase, consisting of two domains connected by a flexible linker which is found in ascomycetes and basidiomycetes. CDH from different producers shows differences with respect to substrate specificity, pH-, and temperature optima.
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Cellobiose dehydrogenase from Sclerotium rolfsii
1988Publisher Summary This chapter describes the assay method and purification procedure of cellobiose dehydrogenase from Sclerotium rolfsii. The chapter also describes the purity, stability, physical, chemical, and enzymatic properties the purified enzyme. The purified enzyme shows one protein band on polyacrylamide gel electrophoresis at pH 2.9 and 8.
Jai C. Sadana, Rajkumar V. Patil
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Cellobiose dehydrogenase production by Sclerotium species pathogenic to plants
Letters in Applied Microbiology, 2002Evaluation of various Sclerotium spp. as producers of the biotechnologically attractive enzyme cellobiose dehydrogenase.All isolates of S. coffeicola, S. delphinii and S. rolfsii grown in shaken flasks on a cellulose-based medium produced appreciable amounts of the extracellular enzyme cellobiose dehydrogenase.Cellobiose dehydrogenase seems to play an ...
R, Ludwig, D, Haltrich
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Cellobiose dehydrogenase from Sporotrichum thermophile
1988Publisher Summary This chapter describes a simple enrichment procedure to obtain cellobiose dehydrogenase preparations virtually free of cellulase using the culture supernatant of Sporotrichum thermophile , an organism producing high yields of this enzyme during growth on cellulose.
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Cellulase assay based on cellobiose dehydrogenase
1988Publisher Summary Most cellulolytic microorganisms (e.g., fungi, aerobic and anaerobic bacteria) bring about the degradation of cellulose by means of different kinds of hydrolytic enzymes that are thought to act in concert on the insoluble substrate producing, at least transiently, different cellodextrins and finally cellobiose.
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Cellobiose dehydrogenase produced by Monilia sp.
1988Publisher Summary This chapter describes the the procedure for the production, partial purification, and characterization of a cellobiose dehydrogenase produced by a species of Monilia . A zymogram method for the detection of cellobiose dehydrogenase activity is also described. Cellobiose dehydrogenase produced by the Monilia sp.
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