Differential response of primary alveolar type I and type II cells to LPS stimulation. [PDF]
The alveolar epithelium serves as a barrier between organism and environment and functions as the first line of protection against potential respiratory pathogens.
Johnson, Meshell D, Wong, Mandi H
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Molluscan cells in culture: primary cell cultures and cell lines [PDF]
In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models.
U Bickham+2 more
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T Cell Development in Culture [PDF]
The T cell compartment is continuously replenished by a renewable source of stem cells. In the adult, bone marrow-derived stem cells seed the thymus and initiate a developmental program that requires a series of incompletely understood signals that are normally provided by the thymus. Failure to recapitulate this process in simple in vitro cultures has
Michael J. Bevan, Sophie M. Lehar
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Assessment of porcine endogenous retrovirus transmission across an alginate barrier used for the encapsulation of porcine islets [PDF]
BACKGROUND: Subcutaneous implantation of a macroencapsulated patch containing human allogenic islets has been successfully used to alleviate type 1 diabetes mellitus (T1DM) in a human recipient without the need for immunosuppression.
Crossan, Claire+4 more
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Profound Re-Organization of Cell Surface Proteome in Equine Retinal Pigment Epithelial Cells in Response to In Vitro Culturing [PDF]
The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. The RPE plays an essential role in visual function and represents the outer blood-retinal barrier.
Aisenbrey+54 more
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Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin [PDF]
Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were used to investigate the presence of these filaments by indirect immunofluorescence microscopy in cultured and non-cultured amniotic fluid cells, in frozen sections ...
Cremer, Marion+4 more
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Exposure of a tendon extracellular matrix to synovial fluid triggers endogenous and engrafted cell death: A mechanism for failed healing of intrathecal tendon injuries [PDF]
Aim: The purpose of this study was to investigate the effect of normal synovial fluid (SF) on exposed endogenous tendon-derived cells (TDC) and engrafted mesenchymal stem cells (MSCs) within the tendon extracellular matrix. Methods: Explants from equine
Dudhia, J+3 more
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Microsystems for Cell Cultures
Microfabricated systems are increasingly being utilized in biotechnological, biomedical, and pharmaceutical research and development as replacements for traditional in vitro cell cultures, bioreactors, and animal experiments (Figure 1) [...]
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Cell isolation and culture [PDF]
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui Zhang, Jeffrey R. Kuhn
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Neural networks on chemically patterned "cultured probe" electrode arrays: network growth and activity patterns [PDF]
A 'cultured probe' is a hybrid type of neural information transducer or prosthesis, for stimulation and/or recording of neural activity in the brain or the spinal cord (ventral motor region or dorsal sensory region).
Buul, B.R.M. van+3 more
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