Tolerance to mutations in the foot-and-mouth disease virus integrin-binding RGD region is different in cultured cells and in vivo and depends on the capsid sequence context. [PDF]
, 2008 Engineered RNAs carrying substitutions in the integrin receptor-binding Arg-Gly-Asp (RGD) region of foot-and-mouth disease virus (FMDV) were constructed (aa 141-147 of VP1 capsid protein) and their infectivity was assayed in cultured cells and suckling
Baranowski, Eric +4 more
core +3 more sources
Liposomal delivery of hydrophobic RAMBAs provides good bioavailability and significant enhancement of retinoic acid signalling in neuroblastoma tumour cells [PDF]
, 2020 Retinoid treatment is employed during residual disease treatment in neuroblastoma, where the aim is to induce neural differentiation or death in tumour cells.
Bilip, Maja +11 more
core +4 more sources
Homogenization of Mammalian Cultured Cells
The Scientific World Journal, 2002 Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any ...
John Graham
doaj +1 more source
ImmunoFISH for Adherent Cultured Mammalian Cells
Bio-Protocol, 2013 This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Francesca Rossiello +2 more
doaj +1 more source
Microelectrode arrays of diamond-insulated graphitic channels for real time detection of exocytotic events from cultured chromaffin cells and slices of adrenal glands [PDF]
, 2016 A microstructured graphitic 4x4 multielectrode array was embedded in a single crystal diamond substrate (4x4 {uG-SCD MEA) for real-time monitoring of exocytotic events from cultured chromaffin cells and adrenal slices.
Battiato, A. +7 more
core +3 more sources
Microgravity-induced apoptosis in cultured glial cells
European Journal of Histochemistry, 2010 Apoptosis is a form of naturally occurring cell death that plays fundamental roles during embryonic developement. In adults, it neatly disposes of cells damaged by injuries provoked by external causes such as UV radiation, ionisation and heat shock ...
BM Uva +6 more
doaj +1 more source
Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices [PDF]
, 2016 The recent success of olfactory ensheathing cell (OEC) assisted regeneration of injured spinal cord has seen a rising interest in the use of these cells in tissue-engineered systems.
Foster, LJR, Green, RA, Hassarati, RT
core +1 more source
Lethal impacts of cigarette smoke in cultured tobacco cells
Tobacco Induced Diseases, 2011 Background In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial.
Masaru Yukihiro +2 more
doaj +1 more source
A method for isolating and culturing placental cells from failed early equine pregnancies [PDF]
, 2016 Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases.
A.J. McGladdery +31 more
core +2 more sources
Primary osteoblast-like cells from patients with end-stage kidney disease reflect gene expression, proliferation, and mineralization characteristics ex vivo. [PDF]
, 2015 Osteocytes regulate bone turnover and mineralization in chronic kidney disease. As osteocytes are derived from osteoblasts, alterations in osteoblast function may regulate osteoblast maturation, osteocytic transition, bone turnover, and skeletal ...
Bowen, Richard E +6 more
core +2 more sources