Results 41 to 50 of about 1,486,027 (268)

Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State

Biological Procedures Online, 2004
Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative.
Corsini Joe, Hacker Christy, Bare Charles   +2 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Extraction and purification of tumorigenic Schwann cells in vitro from neurofibromatosis type 2 tissues

Chinese Journal of Contemporary Neurology and Neurosurgery, 2011
Objective To establish a simple method to extract and purify tumorigenic Schwann cells (TSCs) from neurofibromatosis type 2 (NF2) tumor tissues in vitro, in order to improve the successful rate of primary culture and obtain a large number of TSCs from ...
Wei WANG, Xuejun YANG, Yu LI, Xuetao DONG, Huamin WANG, Haolang MING, Bin ZHANG, Shengping YU   +7 more
doaj  

ASIC currents in cultured primate retinal amacrine/ganglion cells

Physiological Reports
Acid‐sensing ion channels (ASICs) are proton‐gated cation channels belonging to the epithelial Na + channel/degenerin superfamily. In the CNS, ASICs are involved in synaptic plasticity, learning/memory, and acidosis‐mediated injury.
Talib Saafir, Tiandong Leng, Koichi Inoue, Zhi‐Gang Xiong, Peter MacLeish   +4 more
doaj   +1 more source

Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay

Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2021
Objective: To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes.
Nilton Cesar Nogueira dos Santos, Bruno Marques Soares, Cláudia do Ó Pessoa, Livia Maria Andrade de Freitas, João Pedro Pedrosa Cruz, Maria Emília Santos Pereira Ramos, Matheus Melo Pithon, Eneida Moraes de Marcílio Cerqueira   +7 more
doaj  

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells

Journal of Applied Oral Science, 2010
This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. METHODS: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as ...
Andreza Maria Fábio Aranha, Elisa Maria Aparecida Giro, Josimeri Hebling, Fernanda Campos Rosetti Lessa, Carlos Alberto de Souza Costa   +4 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Experimental model for establishment of hypoxia in 75 cm² culture flasks Modelo experimental para o estabelecimento de hipóxia em balões de cultura de 75 cm²

Acta Cirúrgica Brasileira, 2004
In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2.
Sidney Mamoru Keira, Lydia Masako Ferreira, Alfredo Gragnani, Ivone da Silva Duarte, Anelisa Bittencourt Campaner, Marcelino de Souza Durão Jr   +5 more
doaj   +1 more source