Results 61 to 70 of about 1,484,807 (268)

Establishment of chicken muscle and adipogenic cell cultures for cultivated meat production

Frontiers in Nutrition
IntroductionCultured meat seeks to replicate the sensory and nutritional attributes l of conventional meat by developing structured muscle tissue using cell culture.
Vanessa Haach, Karine R. D. Silveira, Maíra de A. Peixoto, Ana Paula Passione e Sá, Vanessa Gressler, Vivian Feddern, Adriana M. G. Ibelli, Luciano Paulino Silva, Ana Paula Bastos   +8 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Identificação de micoplasmas pela inibição de crescimento de amostras isoladas de culturas celulares

Revista de Saúde Pública
As culturas celulares devem ser continuamente monitoradas quanto à presença de micoplasmas, pois, embora às vezes eles passem despercebidos, podem causar alterações cromossômicas, interferir na replicação viral, na produção de anticorpos e interferon.
Jorge Timenetsky, Cosue Miyaki, Inácio França Mendes, Edda de Rizzo   +3 more
doaj  

Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells.

PLoS ONE, 2018
The crosstalk between cells is important for differentiation of cells. Murine-derived feeder cells, SNL76/7 feeder cells (SNLs) or mouse primary embryonic fibroblast feeder cells (MEFs) are widely used for culturing undifferentiated human induced ...
Masaki Shoji, Hiroki Minato, Soichiro Ogaki, Masahide Seki, Yutaka Suzuki, Shoen Kume, Takashi Kuzuhara   +6 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Influence of polyamine analogue DENSPM on cell growth of human SNB19 glioblastoma cells

Chinese Journal of Contemporary Neurology and Neurosurgery, 2011
Objective To investigate the influence of polyamine analogue DENSPM (N1, N11-diethylnorspermine) on cell growth of human SNB19 glioblastoma cells. Methods DENSPM was added into the culture medium of SNB19 cells. MTS assay was used to assess cell survival
Ye TIAN, Bin WANG, Shizhao WANG, Zhengjun WEI, Lin SUN, Rongcai JIANG, Jianning ZHANG   +6 more
doaj  

Possible involvement of microtubules and microfilaments of the epididymal epithelial cells in 17beta-estradiol synthesis.

Folia Histochemica et Cytobiologica, 2004
The rat epididymal epithelial cells revealed features of steroidogenic cells and released 17beta-estradiol (E2) into the culture medium. In steroidogenic cells, elements of the cytoskeleton due to their influence on organelle distribution are implicated ...
Lidia Wenda-Rózewicka, Rafał Kurzawa, Barbara Wiszniewska, Mariola Marchlewicz   +3 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source