Results 81 to 90 of about 6,074,414 (310)

Cultured epidermal stem cells in regenerative medicine

Stem Cell Research & Therapy, 2017
Transplantation of cultured epidermal cell sheets (CES) has long been used to treat patients with burns, chronic wounds, and stable vitiligo. In patients with large area burns this can be a life-saving procedure. The ultimate goal, however, is to restore
Catherine J. Jackson, Kim Alexander Tønseth, Tor Paaske Utheim   +2 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

RC-IAL: linhagem celular contínua de rim de coelho - características e substrato para replicação de vírus

Revista de Saúde Pública
Uma linhagem celular de rim de coelho (denominada RC-IAL), que foi isolada em 1976, e que atualmente está na 150ª passagem, teve suas características analisadas. As células apresentaram morfologia semelhante aos fibroblastos desde o início de seu cultivo.
Aurea Silveira Cruz, Cristina Adelaide Figueiredo, Maria Luisa Barbosa, Clelia Helena de O. Martinez, Luís Florêncio Salles-Gomes   +4 more
doaj  

Establishment of chicken muscle and adipogenic cell cultures for cultivated meat production

Frontiers in Nutrition
IntroductionCultured meat seeks to replicate the sensory and nutritional attributes l of conventional meat by developing structured muscle tissue using cell culture.
Vanessa Haach, Karine R. D. Silveira, Maíra de A. Peixoto, Ana Paula Passione e Sá, Vanessa Gressler, Vivian Feddern, Adriana M. G. Ibelli, Luciano Paulino Silva, Ana Paula Bastos   +8 more
doaj   +1 more source

Enzymatic measurement of phosphatidylserine in cultured cells

Journal of Lipid Research, 2012
Phosphatidylserine (PS) is a quantitatively minor membrane phospholipid involved in diverse cellular functions. In this study, we developed a new fluorometric method for measuring PS using combinations of specific enzymes and Amplex Red.
Shin-ya Morita, Sachimi Shirakawa, Yukiko Kobayashi, Keiko Nakamura, Reiko Teraoka, Shuji Kitagawa, Tomohiro Terada   +6 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Identificação de micoplasmas pela inibição de crescimento de amostras isoladas de culturas celulares

Revista de Saúde Pública
As culturas celulares devem ser continuamente monitoradas quanto à presença de micoplasmas, pois, embora às vezes eles passem despercebidos, podem causar alterações cromossômicas, interferir na replicação viral, na produção de anticorpos e interferon.
Jorge Timenetsky, Cosue Miyaki, Inácio França Mendes, Edda de Rizzo   +3 more
doaj  

Influence of polyamine analogue DENSPM on cell growth of human SNB19 glioblastoma cells

Chinese Journal of Contemporary Neurology and Neurosurgery, 2011
Objective To investigate the influence of polyamine analogue DENSPM (N1, N11-diethylnorspermine) on cell growth of human SNB19 glioblastoma cells. Methods DENSPM was added into the culture medium of SNB19 cells. MTS assay was used to assess cell survival
Ye TIAN, Bin WANG, Shizhao WANG, Zhengjun WEI, Lin SUN, Rongcai JIANG, Jianning ZHANG   +6 more
doaj  

Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells.

PLoS ONE, 2018
The crosstalk between cells is important for differentiation of cells. Murine-derived feeder cells, SNL76/7 feeder cells (SNLs) or mouse primary embryonic fibroblast feeder cells (MEFs) are widely used for culturing undifferentiated human induced ...
Masaki Shoji, Hiroki Minato, Soichiro Ogaki, Masahide Seki, Yutaka Suzuki, Shoen Kume, Takashi Kuzuhara   +6 more
doaj   +1 more source