Results 21 to 30 of about 80,710 (121)
ImmunoFISH for Adherent Cultured Mammalian Cells
Bio-Protocol, 2013 This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Francesca Rossiello +2 more
doaj +1 more source
Superior survival of ex vivo cultured human reticulocytes following transfusion into mice
Haematologica, 2017 The generation of cultured red blood cells from stem cell sources may fill an unmet clinical need for transfusion-dependent patients, particularly in countries that lack a sufficient and safe blood supply.
Sabine Kupzig +4 more
doaj +1 more source
Proceedings of the National Academy of Sciences, 2001 Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
openaire +3 more sources
Development and cytotoxic response of two proliferative MDA- MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines [PDF]
, 2017 Triple-Negative Basal-Like tumors, representing 15 to 20% of breast cancers, are very aggressive and with poor prognosis. Targeted therapies have been developed extensively in preclinical and clinical studies to open the way for new treatment strategies.
arxiv +1 more source
Metabolic Regulatory Network Kinetic Modeling with Multiple Isotopic Tracers for iPSCs [PDF]
arXiv, 2023 The rapidly expanding market for regenerative medicines and cell therapies highlights the need to advance the understanding of cellular metabolisms and improve the prediction of cultivation production process for human induced pluripotent stem cells (iPSCs). In this paper, a metabolic kinetic model was developed to characterize underlying mechanisms of
arxiv
Acta Cirúrgica Brasileira, 2004 In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2.
Sidney Mamoru Keira +5 more
doaj +1 more source
FASEB BioAdvances, 2023 Understanding the expected efficacy and safety of a new regenerative therapy requires analysis of the fate of the transplanted cell graft. We have shown that transplantation of autologous cultured nasal epithelial cell sheets onto the middle ear mucosa ...
Yoshiyuki Kasai +4 more
doaj +1 more source
The Effect of Hypoxic and Normoxic Culturing Conditions in Different Breast Cancer 3D Model Systems
Frontiers in Bioengineering and Biotechnology, 2021 The field of 3D cell cultures is currently emerging, and material development is essential in striving toward mimicking the microenvironment of a native tissue.
Andreas Svanström +11 more
doaj +1 more source
A low-cost, user-friendly rheo-optical compression assay to measure mechanical properties of cell spheroids in standard cell culture plates [PDF]
arXiv, 2023 The mechanical characterization of cell spheroids, one of the most widely used 3D biology models in vitro, is a hotspot of current research on the role played by the mechanical response of cells and tissues. The techniques proposed so far in the literature, while providing important scientific insights, require a specialized equipment and technical ...
arxiv
Journal of Applied Oral Science, 2010 This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. METHODS: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as ...
Andreza Maria Fábio Aranha +4 more
doaj +1 more source