Results 21 to 30 of about 12,058,080 (291)
Homogenization of Mammalian Cultured Cells
Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any ...
John Graham
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Lethal impacts of cigarette smoke in cultured tobacco cells
Background In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial.
Masaru Yukihiro+2 more
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In Vitro Implantation Model Using Human Endometrial SUSD2+ Mesenchymal Stem Cells and Myometrial Smooth Muscle Cells [PDF]
Objective: This study evaluated a novel in vitro implantation model using human endometrial mesenchymal stem cells (EMSCs), SUSD2+, and myometrial smooth muscle cells (SMCs) that were co-cultured with mouse blastocysts as the surrogate embryo ...
Marzieh Rahimipour+2 more
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Microsystems for Cell Cultures
Microfabricated systems are increasingly being utilized in biotechnological, biomedical, and pharmaceutical research and development as replacements for traditional in vitro cell cultures, bioreactors, and animal experiments (Figure 1) [...]
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Microgravity-induced apoptosis in cultured glial cells
Apoptosis is a form of naturally occurring cell death that plays fundamental roles during embryonic developement. In adults, it neatly disposes of cells damaged by injuries provoked by external causes such as UV radiation, ionisation and heat shock ...
BM Uva+6 more
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Cell isolation and culture [PDF]
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui Zhang, Jeffrey R. Kuhn
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TRAIL-Mediated Apoptosis in Breast Cancer Cells Cultured as 3D Spheroids
TNF-alpha-related-apoptosis-inducing-ligand (TRAIL) has been explored as a therapeutic drug to kill cancer cells. Cancer cells in the circulation are subjected to apoptosis-inducing factors.
Siddarth Chandrasekaran+4 more
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Three dimensional neuronal cell cultures more accurately model voltage gated calcium channel functionality in freshly dissected nerve tissue. [PDF]
It has been demonstrated that neuronal cells cultured on traditional flat surfaces may exhibit exaggerated voltage gated calcium channel (VGCC) functionality.
Yinzhi Lai, Ke Cheng, William Kisaalita
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Distinct cell stress responses induced by ATP restriction in quiescent human fibroblasts
Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges.
Nirupama Yalamanchili+5 more
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Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
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