Results 31 to 40 of about 12,756,677 (346)

Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effect

open access: yesStem Cell Research & Therapy, 2021
Background Quality and Quantity culture media (QQ culture media) was reported to enhance vasculogenesis and angiogenesis function of mononuclear cells (MNCs) from healthy volunteers.
Nuttapol Chruewkamlow   +11 more
doaj   +1 more source

Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells.

open access: yesJournal of Clinical Investigation, 1989
Endothelium-derived relaxing factor has been recently identified as nitric oxide. The purpose of this study was to determine if vasodilator drugs that generate nitric oxide inhibit vascular smooth muscle mitogenesis and proliferation in culture.
U. Garg, A. Hassid
semanticscholar   +1 more source

Detection and characterization of Sp1 binding activity in human chondrocytes and its alterations during chondrocyte dedifferentiation. [PDF]

open access: yes, 1997
We have detected DNA binding activity for a synthetic oligonucleotide containing an Sp1 consensus sequence in nuclear extracts from human chondrocytes. Changes in the levels of Sp1 oligonucleotide binding activity were examined in nuclear extracts from ...
Dharmavaram, Rita M.   +3 more
core   +2 more sources

Cell Culture [PDF]

open access: yesCell, 2010
Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey.
openaire   +3 more sources

Distinct cell stress responses induced by ATP restriction in quiescent human fibroblasts

open access: yesFrontiers in Genetics, 2016
Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges.
Nirupama Yalamanchili   +5 more
doaj   +1 more source

Three dimensional neuronal cell cultures more accurately model voltage gated calcium channel functionality in freshly dissected nerve tissue. [PDF]

open access: yesPLoS ONE, 2012
It has been demonstrated that neuronal cells cultured on traditional flat surfaces may exhibit exaggerated voltage gated calcium channel (VGCC) functionality.
Yinzhi Lai, Ke Cheng, William Kisaalita
doaj   +1 more source

Cell culture forensics [PDF]

open access: yesProceedings of the National Academy of Sciences, 2001
Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
openaire   +3 more sources

Magnetofection potentiates gene delivery to cultured endothelial cells [PDF]

open access: yes, 2003
Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance
Gloe, Torsten   +4 more
core   +1 more source

ImmunoFISH for Adherent Cultured Mammalian Cells

open access: yesBio-Protocol, 2013
This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Francesca Rossiello   +2 more
doaj   +1 more source

Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin [PDF]

open access: yes, 1981
Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were used to investigate the presence of these filaments by indirect immunofluorescence microscopy in cultured and non-cultured amniotic fluid cells, in frozen sections ...
Cremer, Marion   +4 more
core   +1 more source

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