Results 31 to 40 of about 80,710 (121)
Characterizing steady states of genome-scale metabolic networks in continuous cell cultures [PDF]
PLOS Computational Biology. 13 (11): e1005835 (2017-11-13), 2017 We present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation.
arxiv +1 more source
Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State
Biological Procedures Online, 2004 Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative.
Corsini Joe +2 more
doaj +1 more source
Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells. [PDF]
PLoS ONE, 2018 The crosstalk between cells is important for differentiation of cells. Murine-derived feeder cells, SNL76/7 feeder cells (SNLs) or mouse primary embryonic fibroblast feeder cells (MEFs) are widely used for culturing undifferentiated human induced ...
Masaki Shoji +6 more
doaj +1 more source
Contraction-induced cluster formation in cardiac cell culture [PDF]
, 2007 Evolution of the spatial arrangement of cells in a primary culture of cardiac tissue derived from newborn rats was studied experimentally over extended period. It was found that cells attract each other spontaneously to form a clustered structure over the timescale of several days.
arxiv +1 more source
Development of input connections in neural cultures [PDF]
Proc Natl Acad Sci U S A. 2008 September 16; 105(37): 13758-13763, 2010 We introduce a novel approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to follow the development of the culture and see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to full term.
arxiv +1 more source
Frontiers in Bioscience-LandmarkObjective: The current study aimed to develop an experimental approach for the direct co-culture of three-dimensional breast cancer cells using single-cell RNA sequencing (scRNA-seq ...
Shuqing Yang +11 more
doaj +1 more source
CARS and SHG microscopy to follow the collagen production in living human corneal fibroblasts and mesenchymal stem cells in fibrin gel 3D cultures [PDF]
, 2011 Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. CARS microscopy can detect lipid membranes and droplet compartments in living cells and SHG microscopy enables a strong imaging contrast for molecules ...
arxiv +1 more source
Microgravity-induced apoptosis in cultured glial cells
European Journal of Histochemistry, 2010 Apoptosis is a form of naturally occurring cell death that plays fundamental roles during embryonic developement. In adults, it neatly disposes of cells damaged by injuries provoked by external causes such as UV radiation, ionisation and heat shock ...
BM Uva +6 more
doaj +1 more source
Distinguishing cancerous from non-cancerous cells through analysis of electrical noise [PDF]
, 2007 Since 1984, electric cell-substrate impedance sensing (ECIS) has been used to monitor cell behavior in tissue culture and has proven sensitive to cell morphological changes and cell motility. We have taken ECIS measurements on several cultures of non-cancerous (HOSE) and cancerous (SKOV) human ovarian surface epithelial cells. By analyzing the noise in
arxiv +1 more source