Results 51 to 60 of about 12,058,080 (291)

ASIC currents in cultured primate retinal amacrine/ganglion cells

Physiological Reports
Acid‐sensing ion channels (ASICs) are proton‐gated cation channels belonging to the epithelial Na + channel/degenerin superfamily. In the CNS, ASICs are involved in synaptic plasticity, learning/memory, and acidosis‐mediated injury.
Talib Saafir, Tiandong Leng, Koichi Inoue, Zhi‐Gang Xiong, Peter MacLeish   +4 more
doaj   +1 more source

Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells

Journal of Applied Oral Science, 2010
This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. METHODS: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as ...
Andreza Maria Fábio Aranha, Elisa Maria Aparecida Giro, Josimeri Hebling, Fernanda Campos Rosetti Lessa, Carlos Alberto de Souza Costa   +4 more
doaj   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source

Extraction and purification of tumorigenic Schwann cells in vitro from neurofibromatosis type 2 tissues

Chinese Journal of Contemporary Neurology and Neurosurgery, 2011
Objective To establish a simple method to extract and purify tumorigenic Schwann cells (TSCs) from neurofibromatosis type 2 (NF2) tumor tissues in vitro, in order to improve the successful rate of primary culture and obtain a large number of TSCs from ...
Wei WANG, Xuejun YANG, Yu LI, Xuetao DONG, Huamin WANG, Haolang MING, Bin ZHANG, Shengping YU   +7 more
doaj  

Experimental model for establishment of hypoxia in 75 cm² culture flasks Modelo experimental para o estabelecimento de hipóxia em balões de cultura de 75 cm²

Acta Cirúrgica Brasileira, 2004
In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2.
Sidney Mamoru Keira, Lydia Masako Ferreira, Alfredo Gragnani, Ivone da Silva Duarte, Anelisa Bittencourt Campaner, Marcelino de Souza Durão Jr   +5 more
doaj   +1 more source

Cell culture

, 1996
This chapter provides the tools for using cell culture as an adjunct to the cell biology and molecular biology methods that break down the traditional borders between the disciplines of biology and virology. Cell culture allows looking at cells in their entirety from the outside before examining the intricacy of their component parts. Cells are counted
George, V.G., Hierholzer, J.C., Ades, E.W.   +2 more
openaire   +2 more sources

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

The nicotinamide hypothesis revisited—plant defense signaling integrating PARP, nicotinamide, nicotinic acid, epigenetics, and glutathione

FEBS Letters, EarlyView.
Nicotinamide (NIC) and nicotinic acid (NIA) are proposed as stress signaling compounds in plants. Oxidative stress may lead to single strand breaks (SSB) in DNA, which activate poly(ADP‐ribose) polymerase (PARP). NIC and NIA are then formed from NAD. NIC and NIA can promote epigenetic changes leading to the expression of defense genes specific for the ...
Torkel Berglund, Anna B. Ohlsson
wiley   +1 more source

Short-term temperature change detections and frequency signals in single cultured cells using a microfabricated thermistor

Sensing and Bio-Sensing Research, 2020
Thermal detection is an effective means of investigating the thermodynamics, metabolic mechanisms, and functions of cells. Here, a sensing device for performing thermal measurements in single cultured cells is fabricated, and its characteristics are ...
Naoki Inomata, Ryohei Inaoka, Kohki Okabe, Takashi Funatsu, Takahito Ono   +4 more
doaj   +1 more source

Single cis‐elements in brassinosteroid‐induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA‐binding domain

FEBS Letters, EarlyView.
Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki, Masae Ohtsuka, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa   +4 more
wiley   +1 more source