Results 91 to 100 of about 358,177 (298)

Gene and library synthesis without amplification: polymerase step reaction (PSR)

open access: yesBioTechniques, 2015
Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene
Zhuo-Bin Lee   +3 more
doaj   +1 more source

UME6, a novel filament-specific regulator of Candida albicans hyphal extension and virulence [PDF]

open access: yes, 2008
Publications prior to 2012 under name Thompson, D.S.Peer ...
Monteagudo, Carlos   +7 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

Cryosectioning and immunofluorescence of C. elegans reveals endogenous polyphosphate in intestinal endo-lysosomal organelles

open access: yesCell Reports: Methods
Summary: Polyphosphate (polyP) is a ubiquitous polyanion present throughout the tree of life. While polyP’s widely varied functions have been interrogated in single-celled organisms, little is known about the cellular distribution and function of polyP ...
Ellen Quarles   +7 more
doaj   +1 more source

The extracellular matrix of plants: Molecular, cellular and developmental biology [PDF]

open access: yes, 1996
A symposium entitled ``The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology was held in Tamarron, Colorado, March 15--21, 1996.

core  

There is more than one way to turn a spherical cellular monolayer inside out: type B embryo inversion in Volvox globator [PDF]

open access: yes, 2011
Höhn S, Hallmann A. There is more than one way to turn a spherical cellular monolayer inside out: type B embryo inversion in Volvox globator. BMC Biology.
Höhn Stephanie   +5 more
core   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

open access: yesFEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani   +3 more
wiley   +1 more source

Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems

open access: yesNature Communications
Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized ...
Yeran Bai   +6 more
doaj   +1 more source

miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation

open access: yesSkeletal Muscle, 2019
Background Myogenesis is driven by specific changes in the transcriptome that occur during the different stages of muscle differentiation. In addition to controlled transcriptional transitions, several other post-transcriptional mechanisms direct muscle ...
Kristen K. Bjorkman   +4 more
doaj   +1 more source

Identification and expression of voltage-gated calcium channel Β subunits in Zebrafish [PDF]

open access: yes, 2008
Voltage-gated calcium channels (VGCC) play important roles in electrically excitable cells and embryonic development. The VGCC Β subunits are essential for membrane localization of the channel and exert modulatory effects on channel functions. In mammals,
John Y. Kuwada   +7 more
core   +1 more source

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