Results 101 to 110 of about 358,177 (298)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Precision multiplexed base editing in human cells using Cas12a-derived base editors

Nature Communications
Base editors enable the direct conversion of target nucleotides without introducing DNA double strand breaks, making them a powerful tool for creating point mutations in a human genome. However, current Cas9-derived base editing technologies have limited
Anabel Y. Schweitzer, Etowah W. Adams, Michael T. A. Nguyen, Monkol Lek, Farren J. Isaacs   +4 more
doaj   +1 more source

Three serendipitous pathways in E. coli can bypass a block in pyridoxal‐5′‐phosphate synthesis

Molecular Systems Biology, 2010
Bacterial genomes encode hundreds to thousands of enzymes, most of which are specialized for particular functions. However, most enzymes have inefficient promiscuous activities, as well, that generally serve no purpose.
Juhan Kim, Jamie P Kershner, Yehor Novikov, Richard K Shoemaker, Shelley D Copley   +4 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Echinoderms : Focus on the Sea Urchin Model in Cellular and Developmental Biology

, 2021
Echinoderms, a sister group of chordates, is a group of exclusively marine animals. TheEchinodermata is an ancient phylum dating to at least 450 million years old and includingmore than 10,000 extant species present throughout the world’s oceans. The modernechinoderms belong to five classes: Echinoidea (e.g., sea urchins and sand dollars),Asteroidea (e.
Pontheaux, Florian, Roch, Fernando, Morales, Julia, Cormier, Patrick   +3 more
openaire   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Genome-wide discovery of structured noncoding RNAs in bacteria

BMC Microbiology, 2019
Background Structured noncoding RNAs (ncRNAs) play essential roles in many biological processes such as gene regulation, signaling, RNA processing, and protein synthesis. Among the most common groups of ncRNAs in bacteria are riboswitches.
Shira Stav, Ruben M. Atilho, Gayan Mirihana Arachchilage, Giahoa Nguyen, Gadareth Higgs, Ronald R. Breaker   +5 more
doaj   +1 more source

Actomyosin-Mediated Cellular Tension Drives Increased Tissue Stiffness and β-Catenin Activation to Induce Epidermal Hyperplasia and Tumor Growth [PDF]

, 2011
Tumors and associated stroma manifest mechanical properties that promote cancer. Mechanosensation of tissue stiffness activates the Rho/ROCK pathway to increase actomyosin-mediated cellular tension to re-establish force equilibrium.
Strachan, D., June Munro, Weaver, Valerie M, Samuel, M., Strachan, David, Weaver, V., Lopez, Jose I., Lopez, J.I., McGhee, Ewan J, Barker, N., Anderson, Kurt I, Schröder, E., Samuel, Michael S, McGhee, E., Nick Barker, McGhee, E.J., Ewan J. McGhee, Sansom, O.J., Brunton, Valerie G., Barker, Nick, Jose I. Lopez, Samuel, Michael S., Timpson, P., Brunton, V., Olson, Michael F, Munro, June, Sansom, Owen J., Croft, D.R., McGhee, Ewan J., Schröder, Ewald, Michael F. Olson, Valerie M. Weaver, Weaver, Valerie M., Ewald Schröder, Daniel R. Croft, Jing Zhou, Sansom, Owen J, Croft, Daniel R., Clevers, Hans, Brunton, Valerie G, Weaver, V.M., Hans Clevers, Olson, M.F., Paul Timpson, David Strachan, Samuel, M.S., Anderson, K.I., Lopez, Jose I, Owen J. Sansom, Anderson, Kurt I., Munro, J., Timpson, Paul, Clevers, H., Olson, Michael F., Croft, D., Lopez, J., Anderson, K., Zhou, J., Sansom, O., Brunton, V.G., Zhou, Jing, Olson, M., Croft, Daniel R, Kurt I. Anderson, Michael S. Samuel, Valerie G. Brunton   +65 more
core   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source