Results 121 to 130 of about 1,059,847 (263)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Characterization of the Cellular Immune Response to Group B Streptococcal Vaginal Colonization. [PDF]

J Innate Immun
Spencer BL, Nguyen DT, Marroquin SM, Gapin L, O'Brien RL, Doran KS.   +5 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Humoral and cellular immune response after COVID-19 vaccination in patients with sickle cell disease on hydroxyurea. [PDF]

Blood Adv
Haggenburg S, Pothast CR, Hofsink Q, Haverkate NJE, Bhoekhan MS, Claireaux M, van Binnendijk RS, Hartog GD, Lissenberg-Witte BI, de Vries RD, van Tuijn C, Biemond BJ, Nur E, Geurts van Kessel CH, Mutsaers PGNJ, Broers AEC, Goorhuis A, Nijhof IS, van Gils MJ, Heemskerk MHM, Hazenberg MD, Rutten CE, van Meerloo JJH, Smit GP, Wouters D, van Leeuwen EMM, Bontkes HJ, Kootstra N, Kant IMJ, Graas T, Toussaint B, de Jong S, Darwesh S, Mahes SS, Kamminga D, Koelewijn M, Faber G, Beaumont G, Engel MD, Pierie RCN, Janssen SR, Faber G, Groen K, Burger JA, Bouhuijs JH, Baars PA, van Dijkman E, Heijmans J, Witte YY, Nahui Palomino RA, Omar SZ, Zweegman S, Kater AP, van den Vegt C, Arends-Halbesma I, de Pater E, Dijkstra MJ, van Beek J, Rots NY, Rijnstra ES, de Rooij DM, Sanders RW, Poniman M, Olijhoek W, van Rijswijk J, Beaumont T, Çetinel L, Schellekens L, den Hartogh YM, Cloos J, Weijers SS, Tonouh-Aajoud S, Avci S, Roelandse-Koop E, Dik WA, Vogels-Nooijen S.   +75 more
europepmc   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Neopterin as a Biomarker of Cellular Immune Response in Renal Allograft Rejection Subtypes: Linking Cytokines and Immune Cells to Improve Diagnostic and Therapeutic Approaches. [PDF]

Biomedicines
Dhital R, Minz M, Minz RW, Anand S, Nada R, Singh S, Kenwar DB, Sharma A.   +7 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Humoral and Cellular Immune Response in Patients with Hematological Disorders After Three Doses of mRNA COVID-19 Vaccine: A Single-Center Observational Study. [PDF]

Vaccines (Basel)
Daffini R, Zecchini F, Venneri G, Malagola M, Cattaneo C, Calza S, Caruso A, Tucci A, Giagulli C.   +8 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

mTOR signaling regulates demand-adapted hematopoiesis and metabolic reprogramming required for an effective cellular immune response in Drosophila melanogaster larvae. [PDF]

PLoS Genet
Anderl I, Ekström JO, Tuomela T, Rämet M, Salminen TS, Vesala L.   +5 more
europepmc   +1 more source