Results 51 to 60 of about 9,566 (219)

Separation of ochratoxins by centrifugal partition chromatography

Journal of Chromatography A
The present research work was dedicated to developing an efficient method based on liquid-liquid chromatography (centrifugal partition chromatography, CPC) applicable to routine purifications of ochratoxins (OT) from the liquid culture of the strain A. albertensis SZMC 2107.
Zsófia Hegedüs, Csilla Gömöri, Mónika Varga, Csaba Vágvölgyi, András Szekeres   +4 more
openaire   +3 more sources

The Terminal Domains of Collagen‐Like Silk Direct Molecular Interactions and Liquid–Liquid Phase Separation‐Mediated Material Assembly

Advanced Functional Materials, EarlyView.
Terminal domains of recombinant collagen‐like silk regulate hierarchical self‐assembly across molecular and material scales. We show that terminal domains of collagen‐like silk enhance triple‐helix stability and drive liquid–liquid phase separation, enabling reversible formation of fibers and self‐healing films.
Mengjie Shen, Daniil Astapov, Nur Alia Oktaviani, Mami Goto, Keiji Numata, A. Sesilja Aranko   +5 more
wiley   +1 more source

Isolation of N-Ethyl-2-pyrrolidinone-Substituted Flavanols from White Tea Using Centrifugal Countercurrent Chromatography Off-Line ESI-MS Profiling and Semi-Preparative Liquid Chromatography

Molecules, 2021
N-Ethyl-2-pyrrolidinone-substituted flavanols (EPSF) are marker compounds for long-term stored white teas. However, due to their low contents and diasteromeric configuration, EPSF compounds are challenging to isolate.
Weidong Dai, Maria Ramos-Jerz, Dongchao Xie, Jiakun Peng, Peter Winterhalter, Gerold Jerz, Zhi Lin   +6 more
doaj   +1 more source

Purification of Nanoparticles by Size and Shape [PDF]

, 2016
Producing monodisperse nanoparticles is essential to ensure consistency in biological experiments and to enable a smooth translation into the clinic. Purification of samples into discrete sizes and shapes may not only improve sample quality, but also ...
A Blanazs, AG Rinzler, AH Latham, B Xiong, BD Chithrani, BM Discher, C Pegoraro, DD Lasic, DE Owens, DL Iden, E Johansson, E Krieg, G Battaglia, G Battaglia, G Battaglia, G Battaglia, G Battaglia, G Battaglia, G Battaglia, G-T Wei, GS Duesberg, J-S Lee, J-W Yoo, JD Robertson, JZ Du, L Messager, M Avila-Olias, M Hanauer, P Satzer, R Bleul, RT Pearson, SF Sweeney, SJ Hurst, T Ruysschaert, V Sharma, V Sánchez-López, X Sun   +36 more
core   +3 more sources

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

Psoralen and ultraviolet A light treatment directly affects phosphatidylinositol 3-kinase signal transduction by altering plasma membrane packing [PDF]

, 2016
Psoralen and ultraviolet A light (PUNTA) are used to kill pathogens in blood products and as a treatment of aberrant cell proliferation in dermatitis, cutaneous T-cell lymphoma, and graft versus-host disease.
Compernolle, Veerle, Devloo, Rosalie, Feys, Hendrik, Sandra, Koen, t'Kindt, Ruben, Van Aelst, Britt, Vandekerckhove, Philippe, Zachée, Pierre   +7 more
core   +2 more sources

Endocytic Control of Cell‐Autonomous and Non‐Cell‐Autonomous Functions of p53

Advanced Science, EarlyView.
NUMB Ex3‐containing isoforms localize to the plasma membrane, where they recruit p53 through SNX9 and direct it to multivesicular bodies and exosomes. Exported p53 is taken up by neighboring cells and activates nuclear programs, revealing an intercellular, exosome‐based pathway that might help establish a tumor‐suppressive microenvironment.
Roberta Cacciatore, Andrea Basile, Stefano Freddi, Irene Schiano Lomoriello, Carlo Ribelle Zucca, Giuseppe Ciossani, Luigi Scietti, Alessandro Cuomo, Simona Ronzoni, Simone Pelicci, Mario Faretta, Elena Zaccheroni, Giuliana Pelicci, Vittoria Matafora, Angela Bachi, Rosalind Helen Gunby, Salvatore Pece, Sara Sigismund, Letizia Lanzetti, Ivan Nicola Colaluca, Pier Paolo Di Fiore   +20 more
wiley   +1 more source

Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium ( sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography

Natural Product Communications, 2015
Aeruginosin-865 was isolated from cultivated soil cyanobacteria using a combination of centrifugal partition chromatography (CPC) and gel permeation chromatography.
José Cheel, Mirjana Minceva, Petra Urajová, Rabya Aslam, Pavel Hrouzek, Jiři Kopecký   +5 more
doaj   +1 more source

Exploration of isoxanthohumol bioconversion from spent hops into 8-prenylnaringenin using resting cells of Eubacterium limosum [PDF]

, 2020
Hops is an almost unique source of the potent phytoestrogen 8-prenylnaringenin (8-PN). As hops contain only low levels of 8-PN, synthesis may be more attractive than extraction.
Bolca, Selin, Goeman, Jan, Moens, Esther, Possemiers, Sam, Van de Wiele, Tom, Van Landschoot, Anita, Verstraete, Willy   +6 more
core   +2 more sources

Nanoscale Spatial Organization of ARC High‐ and Low‐Order Assemblies at Excitatory Synapses

Advanced Science, EarlyView.
ARC (Activity‐Regulated Cytoskeleton‐Associated protein) mediates synaptic plasticity by forming nanoscale assemblies in neurons. Using super‐resolution microscopy and time‐resolved anisotropy with targeted tagging, the study reveals low‐order ARC assemblies at synapses colocalizing with AMPARs, semi‐circular structures at endocytic zones, and 60–80 nm
Martina Damenti, Giovanna Coceano, Mariline Mendes Silva, Jonatan Alvelid, Chiara Sgattoni, Andrea Volpato, Lea Rems, Luciano A. Masullo, Eduard M. Unterauer, Rafal Kowalewski, Lucie Delemotte, Erdinc Sezgin, Ralf Jungmann, Ilaria Testa   +13 more
wiley   +1 more source