Results 91 to 100 of about 240,760 (305)
Journal of Lipid Research, 1982 A simple procedure for coating the interior surface of Beckman Ultra-Clear centrifuge tubes with polyvinyl alcohol is described. The coated tubes are wettable and allow salt solutions to gravity-feed down their sides.
L Holmquist
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Ciência Animal Brasileira, 2015 The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio.
Max Vitória Resende +5 more
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FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Preparation of Crude Subcellular Fractions by Differential Centrifugation
The Scientific World Journal, 2002 The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
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FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
, 2010 Contains fulltext : 97991.pdf (Publisher’s version ) (Open Access)A two-step gradient density centrifugation system has been set up to isolate two contrasting sperm populations of normozoospermic and oligoasthenoteratozoospermic (OAT ...
L. Ramos +9 more
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Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Density separation of quiescent yeast using iodixanol
BioTechniques, 2017 As yeast are starved of nutrients, they enter G0, a quiescent state. Quiescent yeast (Q) cells retain viability for extended periods of time and resume growth following supplementation of missing nutrients.
Ishtiaque Quasem +3 more
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Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation
, 2016 Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are ...
core