Results 251 to 260 of about 240,760 (305)
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Density-Gradient Centrifugation
1998In some cases, further separation of virus from the plant contaminants can be achieved by placing the clarified extract on top of a small volume of a sucrose solution in a centrifuge tube, the so-called sucrose cushion. Although the higher viscosity of the sucrose solution delays the virions reaching the bottom, the plant contaminants may be slowed ...
Jeanne Dijkstra, Cees P. de Jager
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Computer Simulation of Density-Gradient Centrifugation
1979Modern biochemical techniques are continuously refined to produce new and improved results. Improvements in the useful power of a biochemical technique can in principle be achieved in two ways. One is the classic trial-and-error approach whereby the parameters of the experimental conditions are varied one by one until a suitable set of conditions is ...
J, Steensgaard, N P, Møller
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Separation of cytoplasmic particles by centrifugation in a density-gradient
Experientia, 1953Erste Versuche zur Ultrazentrifugierung isolierter Zytoplasmapartikelfraktionen aus Krallenfroschleber (Xenopus laevis Daud.) in «Diodon»-Saccharose-Gradienten mit verschiedenen Dichtebereichen zeigen, dass mitochondrien- und mikrosomenartige Zellpartikel in verschiedenen Zonen der Gradienten ins Dichtegleichgewicht gebracht werden konnen. Die Lage der
H, HOLTER, M, OTTESEN, R, WEBER
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Centrifugation of Liver Microsomes on Metrizamide Density Gradients
Preparative Biochemistry, 1976Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
H, Fehrnström +2 more
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Zonal separations by density-gradient centrifugation
Archives of Biochemistry and Biophysics, 1953Summary Zonal separations in the centrifuge have been obtained by floating a layer of a solution of particles on a second solution which has a density gradient and in which the particles are soluble. During subsequent centrifugation all those particles of the same size, shape, and density sedimented as a zone.
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An Analysis of Fish Genomes by Density Gradient Centrifugation
European Journal of Biochemistry, 1980DNA was prepared from 33 species of fishes representing 12 of the 31 orders of Teleostei and one order of Chondrichthyes. DNA samples were investigated to determine modal and mean buoyant densities in CsCl (q0 and 〈q〉), main‐band asymmetry, intermolecular compositional heterogeneity and base composition.
A P, Hudson +4 more
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Density gradient centrifugation in angle-head rotors
Analytical Biochemistry, 1964Abstract By utilizing DNA and T3 bacteriophage as test materials, good isopyenic banding in CsCl was obtained in #30 and #40 Spinco fixed-angle rotors. Values for the buoyant densities of the materials tested agree with published values and density gradients formed after prolonged centrifugation approximate the calculated compositional gradient at ...
W D, FISHER, G B, CLINE, N G, ANDERSON
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Differentiation of reoviruses and picornaviruses by density gradient centrifugation
Archiv f�r die gesamte Virusforschung, 1967The two HA moieties of reovirus (infectious particle and top component) were recentrifuged in a CsCl gradient. They were stable under these conditions and purified to such an extent that the top component HA moiety contained only 4 TCD50 per unit. Mixtures of poliovirus and reovirus (all three prototypes) were centrifuged in a CsCl density gradient ...
R, Engler, M, Taher, A, Fouad
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Density Gradient Centrifugation of a Murine Leukemia Virus
Science, 1964The Rauscher leukemia virus separated as a single band upon density gradient centrifugation in cesium chloride, rubidium chloride, sucrose, potassium citrate, or potassium tartrate. Prolonged exposure to concentrated potassium citrate or potassium tartrate solutions caused lysis of the virus; the resulting products, having different densities ...
O'conner, T E +2 more
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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation
2023Sophisticated organelle fractionation strategies were the workhorse of early peroxisome research and led to the characterization of the principal functions of the organelle. However, even in the era of molecular biology and "omics" technologies, they are still of importance to unravel peroxisome-specific proteomes, confirm the localization of still ...
Andreas, Manner, Markus, Islinger
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