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Cell therapy manufacturing at full clinical scale: enhancing the quality CAR-T cell therapy starting materials through massively parallel automated microfluidic cell sorting.

Lab Chip
Skelley AM, Behmardi Y, Peterson LF, Inglis DW, Shehada M, Ouaguia L, Gandhi K, Campos-González R, Ward T.   +8 more
europepmc   +1 more source

Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique

Fertility and Sterility, 2009
Objective To compare the effects of density-gradient centrifugation and swim-up on sperm apoptosis by using a multiparameter flow cytometric method. Design Autocontrolled split-sample study. Setting Tertiary infertility center.
Giuseppe Ricci, Sandra Perticarari, Marcella Montico   +2 more
exaly   +2 more sources

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Centrifugation of brain microsomes in a density gradient

Experimental Cell Research, 1960
Abstract Microsomal preparations from rat brain were centrifuged in various density gradients obtained with different concentrations of sucrose. The different zones of the gradients were analyzed chemically as to their protein and RNA content and examined with the electron microscope as to their content of particles and membrane structures.
V, HANZON, G, TOSCHI
openaire   +2 more sources

The Effects of Overloading in Density-Gradient Centrifugation

European Journal of Biochemistry, 1975
The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
J, Steensgaard, N P, Moller, L, Funding
openaire   +2 more sources

Zonal centrifugation in reorienting density gradients

Biochimica et Biophysica Acta (BBA) - General Subjects, 1971
Abstract 1. 1. Zonal rotors which do not require rotating-seal assemblies were tested and employed for quantitative cell fractionations in sucrose density gradients. The design and operation of these rotors represents a departure from other zonal rotors presently in use.
P, Sheeler, D M, Gross, J R, Wells
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Enucleation of cells by density gradient centrifugation

Experimental Cell Research, 1975
Abstract HEp-2 cells can be enucleated by ultracentrifugation in a colloidal silica (PTL) density gradient, containing cytochalasin B. Under optimal conditions, more than 70% of the cells are enucleated. Purification up to 97% is carried out by centrifugation at low speed through a second, preformed PTL density gradient.
W, Bossart, H, Loeffler, K, Bienz
openaire   +2 more sources

Separation of nanorods by density gradient centrifugation

Journal of Chromatography A, 2011
The experimental conditions necessary for the synthesis of well-defined nanoparticles are often difficult to control. There is thus a compelling need for post-synthesis separation of nanoparticles polydispersed in size and shape. We demonstrate here both theoretically and experimentally that gold nanorods with diverse aspect ratios can be separated ...
Bin, Xiong, Jing, Cheng, Yanxia, Qiao, Rui, Zhou, Yan, He, Edward S, Yeung   +5 more
openaire   +2 more sources