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Photometric scanning of centrifuged density gradient columns
Analytical Biochemistry, 1963Abstract Results obtained with an apparatus for recording the ultraviolet absorbance at all depths in a centrifuged density gradient column and for collecting consecutive samples are presented. A heavy sucrose solution is forced from a motor-driven syringe into the bottom of a gradient column.
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The Isolation of Satellite DNA by Density Gradient Centrifugation
2003The term satellite DNA is used for a DNA component that gives a sharp band in a density gradient and can be resolved from the broader main band of DNA in the gradient. The usual gradient material is CsCl in aqueous buffer and the Cs(+) ions form a density gradient in a centrifugal field. DNA in the solution sediments to its isopycnic point. The density
C A, Cooney, H R, Matthews
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The Optimization of Density Gradients for Zonal Centrifugation
Separation Science, 1969Abstract By using established equations and techniques, it is possible to compute sucrose gradients which are optimized to minimize volume expansion of a zone during sedimentation in zonal rotors. These gradients have been tested experimentally, and it has been found that the greatest expansion of the sample zone occurred during the early stage of ...
S. P. Spragg, R. S. Morrod, C. T. Rankin
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Density gradient centrifugation of a graft copolymer
Journal of Polymer Science Part A: General Papers, 1964AbstractA well‐defined graft copolymer of polystyrene and cellulose acetate has been investigated using the density gradient ultracentrifugation method. The initial sample showed two peaks. These were found to be pure graft copolymer and polystyrene; the latter was easily removed by further extraction of the mixture with benzene. The composition of the
H. A. Ende, V. Stannett
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Plant Protoplast Enucleation by Density Gradient Centrifugation
2003A number of different methods have been developed for the enucleation of cells, particularly mammalian cells. Centrifugation of cells adhering to a surface may lead to the nucleus being drawn out from the cytoplasm, resulting in the formation of an enucleated cell or cytoplast and a nucleus surrounded by a thin layer of cytoplasm bounded by a plasma ...
H K, Fakhrai, N, Haq, P K, Evans
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Preparation of polyallomer centrifuge tubes for density gradients
Analytical Biochemistry, 1969Abstract Thin-walled polyallomer centrifuge tubes can easily be prepared to accept linear sucrose gradients, as delivered by conventional gradient formers. They can thus be used with the same facility and achieve the same resolution as cellulose nitrate tubes, for preparative and analytical density gradient centrifugation.
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Isopyknotic cushioning for density gradient centrifugation
Experimental Cell Research, 1959G M, MATEYKO, M J, KOPAC
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