Results 201 to 210 of about 245,556 (263)

Centrifugation of brain microsomes in a density gradient

Experimental Cell Research, 1960
Abstract Microsomal preparations from rat brain were centrifuged in various density gradients obtained with different concentrations of sucrose. The different zones of the gradients were analyzed chemically as to their protein and RNA content and examined with the electron microscope as to their content of particles and membrane structures.
V, HANZON, G, TOSCHI
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The Effects of Overloading in Density-Gradient Centrifugation

European Journal of Biochemistry, 1975
The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
J, Steensgaard, N P, Moller, L, Funding
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Zonal centrifugation in reorienting density gradients

Biochimica et Biophysica Acta (BBA) - General Subjects, 1971
Abstract 1. 1. Zonal rotors which do not require rotating-seal assemblies were tested and employed for quantitative cell fractionations in sucrose density gradients. The design and operation of these rotors represents a departure from other zonal rotors presently in use.
P, Sheeler, D M, Gross, J R, Wells
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Enucleation of cells by density gradient centrifugation

Experimental Cell Research, 1975
Abstract HEp-2 cells can be enucleated by ultracentrifugation in a colloidal silica (PTL) density gradient, containing cytochalasin B. Under optimal conditions, more than 70% of the cells are enucleated. Purification up to 97% is carried out by centrifugation at low speed through a second, preformed PTL density gradient.
W, Bossart, H, Loeffler, K, Bienz
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Separation of nanorods by density gradient centrifugation

Journal of Chromatography A, 2011
The experimental conditions necessary for the synthesis of well-defined nanoparticles are often difficult to control. There is thus a compelling need for post-synthesis separation of nanoparticles polydispersed in size and shape. We demonstrate here both theoretically and experimentally that gold nanorods with diverse aspect ratios can be separated ...
Bin, Xiong, Jing, Cheng, Yanxia, Qiao, Rui, Zhou, Yan, He, Edward S, Yeung   +5 more
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Density-Gradient Centrifugation

1998
In some cases, further separation of virus from the plant contaminants can be achieved by placing the clarified extract on top of a small volume of a sucrose solution in a centrifuge tube, the so-called sucrose cushion. Although the higher viscosity of the sucrose solution delays the virions reaching the bottom, the plant contaminants may be slowed ...
Jeanne Dijkstra, Cees P. de Jager
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Computer Simulation of Density-Gradient Centrifugation

1979
Modern biochemical techniques are continuously refined to produce new and improved results. Improvements in the useful power of a biochemical technique can in principle be achieved in two ways. One is the classic trial-and-error approach whereby the parameters of the experimental conditions are varied one by one until a suitable set of conditions is ...
J, Steensgaard, N P, Møller
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Density Gradient Centrifugation of a Murine Leukemia Virus

Science, 1964
The Rauscher leukemia virus separated as a single band upon density gradient centrifugation in cesium chloride, rubidium chloride, sucrose, potassium citrate, or potassium tartrate. Prolonged exposure to concentrated potassium citrate or potassium tartrate solutions caused lysis of the virus; the resulting products, having different densities ...
O'conner, T E, Rauscher, F J, Zeigel, R F   +2 more
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Centrifugation of Liver Microsomes on Metrizamide Density Gradients

Preparative Biochemistry, 1976
Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
H, Fehrnström, L C, Eriksson, G, Dallner   +2 more
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An analysis of eukaryotic genomes by density gradient centrifugation

Journal of Molecular Biology, 1976
DNA preparations from 25 eukaryotes, ranging from yeast to man, were analyzed in their base composition, sedimentation coefficient, modal and mean buoyant density in CsCl. Four mammalian and two amphibian DNAs were fractionated by preparative Cs 2 SO 4 −Ag + density gradient centrifugation. The CsCl band profile of each fraction was analyzed in terms
Thiery, J, Macaya, G, Bernardi, G
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