Results 211 to 220 of about 245,556 (263)

Zonal separations by density-gradient centrifugation

Archives of Biochemistry and Biophysics, 1953
Summary Zonal separations in the centrifuge have been obtained by floating a layer of a solution of particles on a second solution which has a density gradient and in which the particles are soluble. During subsequent centrifugation all those particles of the same size, shape, and density sedimented as a zone.
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Separation of cytoplasmic particles by centrifugation in a density-gradient

Experientia, 1953
Erste Versuche zur Ultrazentrifugierung isolierter Zytoplasmapartikelfraktionen aus Krallenfroschleber (Xenopus laevis Daud.) in «Diodon»-Saccharose-Gradienten mit verschiedenen Dichtebereichen zeigen, dass mitochondrien- und mikrosomenartige Zellpartikel in verschiedenen Zonen der Gradienten ins Dichtegleichgewicht gebracht werden konnen. Die Lage der
H, HOLTER, M, OTTESEN, R, WEBER
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An Analysis of Fish Genomes by Density Gradient Centrifugation

European Journal of Biochemistry, 1980
DNA was prepared from 33 species of fishes representing 12 of the 31 orders of Teleostei and one order of Chondrichthyes. DNA samples were investigated to determine modal and mean buoyant densities in CsCl (q0 and 〈q〉), main‐band asymmetry, intermolecular compositional heterogeneity and base composition.
A P, Hudson, G, Cuny, J, Cortadas, A E, Haschemeyer, G, Bernardi   +4 more
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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation

2023
Sophisticated organelle fractionation strategies were the workhorse of early peroxisome research and led to the characterization of the principal functions of the organelle. However, even in the era of molecular biology and "omics" technologies, they are still of importance to unravel peroxisome-specific proteomes, confirm the localization of still ...
Andreas, Manner, Markus, Islinger
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Density gradient centrifugation in angle-head rotors

Analytical Biochemistry, 1964
Abstract By utilizing DNA and T3 bacteriophage as test materials, good isopyenic banding in CsCl was obtained in #30 and #40 Spinco fixed-angle rotors. Values for the buoyant densities of the materials tested agree with published values and density gradients formed after prolonged centrifugation approximate the calculated compositional gradient at ...
W D, FISHER, G B, CLINE, N G, ANDERSON
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Differentiation of reoviruses and picornaviruses by density gradient centrifugation

Archiv f�r die gesamte Virusforschung, 1967
The two HA moieties of reovirus (infectious particle and top component) were recentrifuged in a CsCl gradient. They were stable under these conditions and purified to such an extent that the top component HA moiety contained only 4 TCD50 per unit. Mixtures of poliovirus and reovirus (all three prototypes) were centrifuged in a CsCl density gradient ...
R, Engler, M, Taher, A, Fouad
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Density Gradient Centrifugation Studies on Rabies Virus

Journal of Bacteriology, 1966
Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski . Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92: 102–106.
A R, Neurath, T J, Wiktor, H, Koprowski
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Density gradient centrifugations of adenovirus-antibody complexes

Archiv f�r die gesamte Virusforschung, 1965
Buoyant density sedimentations in CsCl of adenovirus type 5 and virus-antibody complexes has been described. It has been demonstrated that virus which survives a certain dose of antibody is found at densities lower than that of untreated particles suggesting a coating of the virus with antibody.
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Isolation of Endocitic Organelles by Density Gradient Centrifugation

2008
Advanced prefractionation strategies, in combination with highly sensitive and accurate mass spectrometers provide powerful means to detect and analyze low abundant proteins on the subcellular and organelle-specific level. Among enrichment techniques, subcellular fractionation has become the most commonly used.
Mariana Eça Guimarães, de Araùjo, Lukas Alfons, Huber, Taras, Stasyk   +2 more
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Isolation of bovine eosinophils by density gradient centrifugation

American Journal of Veterinary Research, 1985
SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092.
W H, Chambers, S M, Washburn, M, Campos, P H, Klesius   +3 more
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