Results 171 to 180 of about 2,428 (190)
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Production and Purification of CGTase of Alkalophylic Bacillus Isolated from Brazilian Soil
Applied Biochemistry and Biotechnology, 1998Alkalophylic bacilli that produce cyclodextringlycosyltransferase (CGTase) were isolated from Brazilian soil, with a scheme of two plating steps. In the first step, the bacterial isolate forms a halo in the cultivation medium that contains gamma-cyclodextrin (CD) complexing dyes.
G, Matioli +3 more
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MOLECULAR DYNAMICS SIMULATION TO ELUCIDATE THE THERMOSTABILITY OF B DOMAIN IN CGTASE.
Theoretical biology forum, 2016Cyclodextrin glycosyltransferase (EC2.4.1.19, CGTase) catalyzes the formation of cyclodextrins from starch. Thermal stability is of great importance for this enzyme. In order to gain better understanding of the thermostability mechanisms of the protein, we herein report structural features of the CGTase B domain at different temperatures.
Fu, Yi, Chen, Zhiguo, Zhao, Ji
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Journal of inclusion phenomena and macrocyclic chemistry, 2002
The enzyme cyclodextringlycosyltransferase (CGTase) was immobilized in commercial chitosan with different methods of immobilization at different temperatures, with the aim of obtaining a product with improved activity and higher recovery of the free enzyme activity.
Keli C.A. Sobral +4 more
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The enzyme cyclodextringlycosyltransferase (CGTase) was immobilized in commercial chitosan with different methods of immobilization at different temperatures, with the aim of obtaining a product with improved activity and higher recovery of the free enzyme activity.
Keli C.A. Sobral +4 more
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Optimization of Enzymatic Reaction Conditions of γ-Cyclomaltodextrin by CGTase
Advanced Materials Research, 2013A strain of bacillus alkalophilus12-7 which could produce glucanotransferase (CGTase) forming γ-cyclodextrin was screened. Cyclodextrin production technology conditions were studied, and we mainly discussed the influence of enzymatic reaction conditions on γ-cyclodextrin production by glucanotransferase.
Xin Zhi Cao, Hong Mei Ming, Li Xiong
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Cyclodextrin producing enzyme (CGTase)
1996Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. By use of the crystal CGTase preparation, the action of the
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Organic solvent and pH induced alteration of product specificity of CGTase
Biotechnology and Bioprocess Engineering, 1998Cyclodextrin glucanotransferase [CGTase, E.C.2.4.1.19] is an extracellular enzyme, which catalyzes the formation of α−, β−, γ− CDs from starch. Their proportions of formations depend on enzyme sources and reaction conditions. To understand what determines the product specificity of CGTases, we examined the alteration of product specificity of CGTase ...
Kyo-Sun Park +4 more
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Enhancing the cyclodextrin production by synchronous utilization of isoamylase and α-CGTase
Applied Microbiology and Biotechnology, 2012Cyclodextrins (CD) are cyclic α-1,4-glucans composed of glucose units, and they have multiple applications in food, pharmaceuticals, cosmetics, agriculture, chemicals, etc. CD are usually produced by cyclodextrin glycosyltransferase (CGTase) from starch.
Xuguo, Duan +3 more
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Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112
Protein Expression and Purification, 2017Cyclodextrin glycosyltransferase (CGTase) is an enzyme able to convert starch and other substrates into cyclodextrins (CDs). A marine strain Y112 producing α-CGTase was identified as Bacillus agaradhaerens Y112 by physiological and biochemical characterization, and 16S rDNA analysis.
Jian-Hua, Hao +6 more
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Carbohydrate Research, 2015
Cyclodextrin glycosyltransferases (CGTase) are reported to selectively catalyze α(1 → 4)-glycosyl transfer reactions besides showing low hydrolytic activity. Here, the effect of the anomeric configuration of the glycosyl acceptor on the regioselectivity of CGTase catalyzed glycosylations was investigated.
Simon, Strompen +4 more
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Cyclodextrin glycosyltransferases (CGTase) are reported to selectively catalyze α(1 → 4)-glycosyl transfer reactions besides showing low hydrolytic activity. Here, the effect of the anomeric configuration of the glycosyl acceptor on the regioselectivity of CGTase catalyzed glycosylations was investigated.
Simon, Strompen +4 more
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Journal of Biotechnology, 2011
Bacillus macerans cyclodextrin glycosyltransferase (CGTase) was used to convert dodecyl-β-maltoside (DDM) to dodecyl-β-maltooctaoside (DDMO) using α-cyclodextrin (α-CD) or starch as glycosyl donors. At 300mM α-CD, varied DDM concentration and 60°C, the reaction obeyed Michaelis-Menten kinetics with a K(m) value of 18mM and a V(max) value of 100U/mg ...
Daniela, Zehentgruber +3 more
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Bacillus macerans cyclodextrin glycosyltransferase (CGTase) was used to convert dodecyl-β-maltoside (DDM) to dodecyl-β-maltooctaoside (DDMO) using α-cyclodextrin (α-CD) or starch as glycosyl donors. At 300mM α-CD, varied DDM concentration and 60°C, the reaction obeyed Michaelis-Menten kinetics with a K(m) value of 18mM and a V(max) value of 100U/mg ...
Daniela, Zehentgruber +3 more
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