Economic model for obtaining cyclodextrins from commercial cgtase [PDF]
Brazilian Journal of Pharmaceutical Sciences, 2021 A repetitive batch process was employed followed by membrane ultrafiltration system to produce low-cost cyclodextrins (CDs) using commercial enzymes Toruzyme® cyclomaltodextrin glucanotransferase (CGTase) and its kinetic parameters were determined.
Luciana Numata Koga +6 more
doaj +3 more sources
Cyclodextrin glycosyltransferase immobilization on functionalized graphene nanoplatelets for enhanced cyclodextrin production. [PDF]
PLoS ONEImmobilization of enzyme on functionalized nanomaterials offers a promising strategy for enhancing enzyme stability, reusability, and activity in a variety of industrial applications.
Babatunde A Ogunbadejo +1 more
doaj +2 more sources
Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity [PDF]
Brazilian Archives of Biology and Technology, 2004 The production of beta- and gamma -CD by CGTase from Bacillus firmus was studied, in regard to the effect of the source and concentration of starch on the yield of CD and CGTase activity.
Ilma Hiroko Higuti +2 more
doaj +5 more sources
Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli [PDF]
BMC Biotechnology, 2018 Background Cyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosmetics, chemicals, and agriculture,
Lei Wang, Sheng Chen, Jing Wu
doaj +3 more sources
Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13. [PDF]
Eng Life Sci, 2022 Abstract Stevia rebaudiana is a sweet herbaceous perennial plant, which is frequently used in the preparation of plant‐based sweeteners. The demand for such sweeteners continues to increase due to purposeful nutrition and modern‐day metabolic syndromes.
Czinkóczky R, Németh Á.
europepmc +2 more sources
Reviewing Glycosyl-Inositols: Natural Occurrence, Biological Roles, and Synthetic Techniques. [PDF]
ChembiochemOverall, this comprehensive review underscores the significance of glycosyl inositols as versatile molecules with diverse biological functions and promising applications in scientific research and industry. Methods for their chemical and enzymatic synthesis are also reviewed, highlighting recent advances and challenges in the field.
Miranda-Molina A +3 more
europepmc +2 more sources
Sustainable and Scalable Enzymatic Production, Structural Elucidation, And Biological Evaluation of Novel Phlorizin Analogues. [PDF]
ChemSusChemNew α‐glycosides of chalcones and dihydrochalcones, analogues of naturally occurring phlorizin, have been synthesized using a CGTase‐catalyzed glycosylation that was successfully scaled‐up in a 10‐liter reactor. The α‐glycosides were (i) purified using an original centrifugal partition chromatography technique, (ii) characterized through NMR and LC–MS ...
Minsat L +12 more
europepmc +2 more sources
Conversion of a cyclodextrin glucanotransferase into an alpha-amylase:Assessment of directed evolution strategies [PDF]
, 2007 Glycoside hydrolase family 13 (GH13) members have evolved to possess various distinct reaction specificities despite the overall structural similarity.
Dijkhuizen, Lubbert +2 more
core +11 more sources
Engineering CGTase to improve synthesis of alkyl glycosides [PDF]
Glycobiology, 2020 Abstract Alkyl glycoside surfactants with elongated carbohydrate chains are useful in different applications due to their improved biocompatibility. Cyclodextrin glucanotransferases can catalyze the elongation process through the coupling reaction.
Kazi Zubaida Gulshan Ara +6 more
openaire +2 more sources
CGTase, a novel antimicrobial protein from Bacillus cereus YUPP-10, suppresses Verticillium dahliae and mediates plant defence responses. [PDF]
Mol Plant Pathol, 2021 Cyclodextrin glycosyltransferase, a hydrolytic protease from Bacillus cereus YUPP‐10, inhibits the growth of Verticillium dahliae and activates cotton innate immunity. The domains for hydrolysis activity and disease resistance induction were identified.
Zhou J +9 more
europepmc +2 more sources