Results 31 to 40 of about 2,428 (190)

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

Enzymatic Synthesis of Phloretin α‐Glucosides Using a Sucrose Phosphorylase Mutant and its Effect on Solubility, Antioxidant Properties and Skin Absorption

Advanced Synthesis &Catalysis, Volume 363, Issue 12, Page 3079-3089, June 21, 2021., 2021
Abstract Glycosylation of polyphenols may increase their aqueous solubility, stability, bioavailability and pharmacological activity. Herein, we used a mutant of sucrose phosphorylase from Thermoanaerobacterium thermosaccharolyticum engineered to accept large polyphenols (variant TtSPP_R134A) to produce phloretin glucosides.
Jose L. Gonzalez‐Alfonso, Zorica Ubiparip, Elena Jimenez‐Ortega, Ana Poveda, Cristina Alonso, Luisa Coderch, Jesus Jimenez‐Barbero, Julia Sanz‐Aparicio, Antonio O. Ballesteros, Tom Desmet, Francisco J. Plou   +10 more
wiley   +1 more source

Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

Biotechnology Reports, 2016
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h).
Suryani Saallah, M. Nazli Naim, I. Wuled Lenggoro, Mohd Noriznan Mokhtar, Noor Fitrah Abu Bakar, Masao Gen   +5 more
doaj   +1 more source

Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 [PDF]

, 2005
A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43
A. Rahman, R, Hassan, O., Ho, Kek Sian, Ismail, A. F., Kamaruddin, K., Md. Illias, R, Nik Mahmood, N. A., Said, M.   +7 more
core   +1 more source

Biokatalyse: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 133, Issue 1, Page 89-123, January 4, 2021., 2021
Die Biokatalyse hat sich zu einer reifen Technologie für die Synthese von Chemikalien und Pharmazeutika entwickelt, wo sie durch hohe Selektivität und milde Reaktionsbedingungen Vorteile bringt. Dieser Aufsatz hebt aktuelle Erfolge hervor, mit besonderem Fokus auf industrielle Anwendungen und führt Leistungsindikatoren ein, um die Effizienz von ...
Shuke Wu, Radka Snajdrova, Jeffrey C. Moore, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Reassessment of Acarbose as a Transition State Analogue Inhibitor of Cyclodextrin Glycosyltransferase [PDF]

, 1998
The binding of several different active site mutants of Bacillus circulans cyclodextrin glycosyltransferase to the inhibitor acarbose has been investigated through measurement of Ki values.
Dijkstra, Bauke W.,, Mosi, Renee,, Ruiterkamp, Richard,, Sham, Howard,, Uitdehaag, Joost C.M.,, Withers, Stephen G.,   +5 more
core   +2 more sources

Biocatalysis: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Volume 60, Issue 1, Page 88-119, January 4, 2021., 2021
Biocatalysis has developed into a mature technology for chemical and pharmaceutical synthesis as well as other areas where high selectivity and mild reaction conditions are required. This Review highlights recent achievements with a special focus on industrialized applications including the introduction of key performance indicators (KPIs) to judge the
Shuke Wu, Radka Snajdrova, Jeffrey C. Moore, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Reaction of CGTase on Amylose.

Journal of Applied Glycoscience, 2001
The action of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) on amylose was reinvesti-gated by determining the product specificity of the cyclization reaction and the substrate specificity of the coupling and hydrolytic reactions of CGTases from alkalophilic Bacillus sp.
Yoshinobu Terada, Haruyo Sanbe, Takeshi Takaha, Shigetaka Okada, Sumio Kitahata, Kyoko Koizumi   +5 more
openaire   +2 more sources

Integral use of amaranth starch to obtain cyclodextrin glycosyltransferase, by Bacillus megaterium, to produce β-cyclodextrin.

Frontiers in Microbiology, 2016
Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrins (CDs) from starch and related carbohydrates, producing a mixture of α-, β-, and γ-CDs in different amounts.
María Belem Arce-Vázquez, Edith Ponce-Alquicira, Ezequiel Delgado-Fornué, Ruth Pedroza-Islas, Gerardo Díaz-Godínez, JORGE SORIANO-SANTOS   +5 more
doaj   +1 more source

Trapping and Characterization of the Reaction Intermediate in Cyclodextrin Glycosyltransferase by Use of Activated Substrates and a Mutant Enzyme [PDF]

, 1997
Cyclodextrin glycosyltransferases (CGTases) catalyze the degradation of starch into linear or cyclic oligosaccharides via a glycosyl transfer reaction occurring with retention of anomeric configuration.
Dijkstra, Bauke W.,, He, Shouming,, Mosi, Renee,, Uitdehaag, Joost,, Withers, Stephen G.,   +4 more
core   +3 more sources