Results 1 to 10 of about 18,484 (195)

Time-resolved spectroscopic and electrophysiological data reveal insights in the gating mechanism of anion channelrhodopsin

Communications Biology, 2021
Channelrhodopsins are widely used in optogenetic applications. High photocurrents and low current inactivation levels are desirable. Two parallel photocycles evoked by different retinal conformations cause cation-conducting channelrhodopsin-2 ( Cr ChR2 ...
Max-Aylmer Dreier, Philipp Althoff, Mohamad Javad Norahan, S. A. Tennigkeit, S. El-Mashtoly, M. Lübben, C. Kötting, T. Rudack, K. Gerwert   +8 more
semanticscholar   +2 more sources

Specific residues in the cytoplasmic domain modulate photocurrent kinetics of channelrhodopsin from Klebsormidium nitens

Communications Biology, 2021
Channelrhodopsins (ChRs) are light-gated ion channels extensively applied as optogenetics tools for manipulating neuronal activity. All currently known ChRs comprise a large cytoplasmic domain, whose function is elusive.
R. Tashiro, Kumari Sushmita, S. Hososhima, Sunita Sharma, S. Kateriya, H. Kandori, S. Tsunoda   +6 more
semanticscholar   +2 more sources

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin

eLife, 2021
Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as ...
K. Oda, Takashi Nomura, T. Nakane, K. Yamashita, Keiichi Inoue, S. Ito, J. Vierock, K. Hirata, A. Maturana, K. Katayama, T. Ikuta, I. Ishigami, T. Izume, R. Umeda, R. Eguma, S. Oishi, G. Kasuya, Takafumi Kato, T. Kusakizako, W. Shihoya, H. Shimada, T. Takatsuji, M. Takemoto, R. Taniguchi, A. Tomita, R. Nakamura, M. Fukuda, H. Miyauchi, Yongchan Lee, E. Nango, Rie Tanaka, Tomoyuki Tanaka, M. Sugahara, Tetsunari Kimura, T. Shimamura, T. Fujiwara, Y. Yamanaka, S. Owada, Y. Joti, K. Tono, R. Ishitani, S. Hayashi, H. Kandori, P. Hegemann, S. Iwata, M. Kubo, T. Nishizawa, O. Nureki   +47 more
semanticscholar   +3 more sources

Atomistic Insight into the Role of Threonine 127 in the Functional Mechanism of Channelrhodopsin-2 [PDF]

Applied Sciences, 2019
Channelrhodopsins (ChRs) belong to the unique class of light-gated ion channels. The structure of channelrhodopsin-2 from Chlamydomonas reinhardtii (CrChR2) has been resolved, but the mechanistic link between light-induced isomerization of the ...
Bamann, Christian, Ehrenberg, David, Heberle, Joachim, Heinrich, Dorothea, Hoffmann, Kirsten, Kar, Rajiv K., Krause, Nils, Saita, Mattia, Schapiro, Igor, Schlesinger, Ramona   +9 more
core   +3 more sources

Dual-color optical activation and suppression of neurons with high temporal precision. [PDF]

Elife
A well-known phenomenon in the optogenetic toolbox is that all light-gated ion channels, including red-shifted channelrhodopsins (ChRs), are activated by blue light, whereas blue-shifted ChRs are minimally responsive to longer wavelengths.
Mermet-Joret N, Moreno A, Zbela A, Nazari M, Ellendersen BE, Baro RC, Krauth N, von Philipsborn A, Sørensen AT, Piriz J, Lin JY, Nabavi S.   +11 more
europepmc   +2 more sources

Channelrhodopsin Excitation Contracts Brain Pericytes and Reduces Blood Flow in the Aging Mouse Brain in vivo

Frontiers in Aging Neuroscience, 2020
Brains depend on blood flow for the delivery of oxygen and nutrients essential for proper neuronal and synaptic functioning. French physiologist Rouget was the first to describe pericytes in 1873 as regularly arranged longitudinal amoeboid cells on ...
Amy R. Nelson, Meghana Sagare, Yaoming Wang, Kassandra Kisler, Z. Zhao, B. Zlokovic   +5 more
semanticscholar   +3 more sources

HulaChrimson: A Chrimson-like cation channelrhodopsin discovered using freshwater metatranscriptomics from Lake Hula. [PDF]

Biophys Physicobiol
Chrimson is cation-conducting channelrhodopsin (CCR) with the most red-shifted absorption spectrum, rendering itself as one of the most promising optogenetic tools.
Takahashi H, Takaramoto S, Nagata T, Fainsod S, Kato Y, Béjà O, Inoue K.   +6 more
europepmc   +2 more sources

Red‐shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina

EMBO Molecular Medicine, 2016
Targeting the photosensitive ion channel channelrhodopsin‐2 (ChR2) to the retinal circuitry downstream of photoreceptors holds promise in treating vision loss caused by retinal degeneration. However, the high intensity of blue light necessary to activate
Abhishek Sengupta, Antoine Chaffiol, Emilie Macé, Romain Caplette, Mélissa Desrosiers, Maruša Lampič, Valérie Forster, Olivier Marre, John Y Lin, José‐Alain Sahel, Serge Picaud, Deniz Dalkara, Jens Duebel   +12 more
semanticscholar   +2 more sources

Ultrafast Backbone Protonation in Channelrhodopsin-1 Captured by Polarization Resolved Fs Vis-pump—IR-Probe Spectroscopy and Computational Methods

Molecules, 2020
Channelrhodopsins (ChR) are light-gated ion-channels heavily used in optogenetics. Upon light excitation an ultrafast all-trans to 13-cis isomerization of the retinal chromophore takes place.
T. Stensitzki, Suliman Adam, R. Schlesinger, I. Schapiro, K. Heyne   +4 more
semanticscholar   +3 more sources

Channelrhodopsins with distinct chromophores and binding patterns. [PDF]

Nat Commun
Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii and H.
Shan Y, Zhao L, Chen M, Li X, Zhang M, Pei D.   +5 more
europepmc   +2 more sources