Results 121 to 130 of about 108,946 (271)

Harnessing Bulk‐Segregant Mapping to Identify Trait‐Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Forward genetics has been instrumental in identifying genes underlying desirable traits, yet its application to polyploid plants, many of which are key agricultural crops, remains challenging due to their genomic complexity. Therefore, we developed BenthMap, a bulk segregant analysis platform for high‐throughput trait mapping and gene ...
Zuba Ahmed, Jiyuan An, Satomi Hayashi, Julia Bally, Chris Winefield, Peter M. Waterhouse   +5 more
wiley   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Beyond Starch: Towards a Scalable Potato Platform for Molecular Farming

Plant Biotechnology Journal, EarlyView.
Re‐engineering potato as a biosafe and host‐optimised platform for plant molecular farming by integrating intrinsic biological traits with targeted engineering strategies. ABSTRACT Thirty‐five years after the first recombinant protein was produced in potato and 30 years after clinical trials of edible vaccines from its tubers, the crop is being ...
Izabela Anna Chincinska, Dorota Sołtys‐Kalina, Audrey Y.‐H. Teh   +2 more
wiley   +1 more source

Structural Elucidation and Engineering of the (S)‐scoulerine 2‐O‐Methyltransferase Enabling Regioselective Epiberberine Biosynthesis in Coptis chinensis

Plant Biotechnology Journal, EarlyView.
ABSTRACT Protoberberine alkaloids are a characteristic group of natural products in Coptis plants known for their notable pharmacological activities. However, the structural similarity and the substrate promiscuity of their biosynthetic enzymes have left the precise synthetic pathways remain unclarified, posing challenges to regulate product formation.
Jun Song, Di Liu, Shi Chen, Yufeng Tan, Siyu Wang, Shihan Li, Yutong Qin, Guofeng Li, Xufang Tian, Lan Yang, Chong Yuan, Junbo Gou, Wei Huang, Shilin Chen, Yifei Liu   +14 more
wiley   +1 more source

Research on commercially electric wheelchairs and improving the running performance of passive link of joystick-type 6-wheel electric wheelchairs on uphill and downhill roads Part 1

Nihon Kikai Gakkai ronbunshu
As the number of elderly people increases, the demand for electric wheelchairs is increasing. Among them is a joystick-type 6-wheel electric wheelchair. It has three wheels, the central wheel of which is the driving wheel in side view.
Tetsuaki KAWATA
doaj   +1 more source

DcH3.3 and DcNAC1 Regulate the Expression of UGT73A93 Involved in the Changes in Flower Colour and Fungal Resistance in Carnation

Plant Biotechnology Journal, EarlyView.
ABSTRACT Carnation (Dianthus caryophyllus) contains abundant flavonoid glycosides (FGs), which are important natural functional and colour components. However, there are few reports on the modification of UDP‐glycosyltransferases (UGTs) in relation to flavonoids in carnation.
Xuhong Zhou, Qirui Xiong, Yue Zhang, Xiumei Yang, Siyuan Zhang, Yanxia Tao, Xiaomi Yang   +6 more
wiley   +1 more source

NUMERICAL STUDY ON THE MATERIAL STRENGTH OF A MICROCAR CHASSIS STRUCTURE UNDER STATIC AND DYNAMIC LOADS

Scientific Journal of Mechanical Engineering Kinematika
The growth of microcars as an urban mobility solution demands an efficient yet strong and safe structural design. This study aims to analyze the structural performance of microcar chassis using a Finite Element Method (FEM)-based numerical simulation ...
Rahmatia, Lizda Johar Mawarani, Riyki Apriandi   +2 more
doaj   +1 more source

Multi‐Omics Analyses Reveal the Red and Far‐Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana

Plant, Cell &Environment, EarlyView.
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang, Fengjiao Wang, Ran Du, Tao Li, Shaoqun Zhou, Jianbin Yan, Wei Li   +6 more
wiley   +1 more source

Sound offset responses become highly informative in the auditory cortex

The Journal of Physiology, EarlyView.
Abstract figure legend Using large datasets of neural responses to diverse sounds recorded across four stages of the mouse auditory system (depicted and colour‐coded on the left), offset responses occurring after the termination of each sound (time window indicated by the orange shaded area superimposed with activity traces) were characterized and it ...
Charly Lamothe, Sophie Bagur, Etienne Gosselin, Brice Bathellier   +3 more
wiley   +1 more source

A FIRST APPROXIMATION CALCULATION OF AIR CUSHION CHASSIS WEIGHT OF TRANSPORT AIRPLANE

Научный вестник МГТУ ГА, 2016
This article describes a first approximation of a weighted estimate of air cushion chassis. The algorithm for calculating the weight of air cushion chassis allows not only to estimate the mass of the chassis to a first approximation, but also to conduct ...
V. P. Morozov, Y. Y. Merzlikin
doaj