Results 81 to 90 of about 15,869,778 (313)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger

Microbial Cell Factories
Despite revolutionizing fungal genetic engineering, conventional CRISPR/Cas9-mediated knockouts rely on DNA double-strand breaks (DSBs), which can cause unwanted insertions and deletions, chromosomal abnormalities, and cytotoxicity.
Guoliang Yuan, Shuang Deng, Ziyu Dai, Beth A. Hofstad, Kyle R. Pomraning   +4 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Antea Group Archeologie 2016/107

, 2017
Op 22 februari 2016 heeft Antea Group in opdracht van de gemeente Hilversum een archeologische begeleiding uitgevoerd ten behoeve van een kleinschalig crematorium op het terrein.
Colijn, MA J.E. (Antea Group)
core   +1 more source

Antea Group Archeolgoie 2019/15

, 2020
Antea Group projectnummer 433403 Antea Group heeft in de 17 juli 2018 tot 3 augustus 2018 een archeologisch veldonderzoek (Opgraving, variant archeologische begeleiding) uitgevoerd aan het Achterom te Groningen.
Akkerman, E. (Antea Group), Sibma, T. (Antea Group), Muller, C. (Antea Group)   +2 more
core   +1 more source

Research on Mechanical Properties of Concrete at High Temperatures Based on Machine Learning

Zhongwai Gonglu
Structural safety is directly affected by the mechanical properties of concrete at high temperatures. Firstly, based on the existing compression and tension test data of concrete at high temperatures, the Abaqus finite element software was adopted for ...
LIU Junhua, LIU Bin, CAO Haifeng, LIU Zhiguang, LI Zhiyong   +4 more
doaj   +1 more source

Safety and efficacy of the feed additive 4-methyl-5-vinylthiazole [15.018] belonging to chemical group 29 for all animal species (FEFANA asbl). [PDF]

EFSA J, 2023
EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Bampidis V, Azimonti G, Bastos ML, Christensen H, Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A, Petkova M, Ramos F, Ramos F, Sanz Y, Villa RE, Woutersen R, Brantom P, Chesson A, Westendorf J, Manini P, Dusemund B.   +22 more
europepmc   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source