Results 81 to 90 of about 320,155 (260)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Statistical physics of self-replication [PDF]

, 2013
Self-replication is a capacity common to every species of living thing, and simple physical intuition dictates that such a process must invariably be fueled by the production of entropy. Here, we undertake to make this intuition rigorous and quantitative
England, Jeremy L., Jeremy L. England
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Core excitations of naphthalene: Vibrational structure versus chemical shifts [PDF]

, 2004
High-resolution x-ray photoelectron emission (XPS) and near-edge x-ray absorption fine structure (NEXAFS) spectra of naphthalene are analyzed in terms of the initial state chemical shifts and the vibrational fine structure of the excitations.
Osikowicz, W., Murdey, R, Ohrwall, G., Osikowicz, Wojciech,, R. Murdey, Agren, H, Braun, S., H. Ågren, Salaneck, W.R., Friedlein, Rainer,, Friedlein, R, Minkov, Ivaylo,, R. Friedlein, Suess, C., Murdey, R., S. L. Sorensen, Osikowicz, W, Gelmukhanov, Faris,, Örhvall, G, C. Suess, Ågren, Hans,, Gel'mukhanov, F., Friedlein, R., Salaneck, William R,, Ristinmaa Sörensen, Stacey, Braun, Slawomir,, Sorensen, S. L., Salaneck, W. R. and Agren, H., F. Gel’mukhanov, Agren, H.,, Braun, S, W. R. Salaneck, W. Osikowicz, G. Öhrwall, Minkov, I.,, Sorensen, S.L.,, Suess, C, Minkov, I, Sorensen, S.L., Ohrwall, G.,, S. Braun, Minkov, I., Salaneck, WR, I. Minkov, Gel'mukhanov, F, Gel'mukhanov, F.,, Ohrwall, G   +46 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Li + ionic conductivities and diffusion mechanisms in Li-based imides and lithium amide [PDF]

, 2012
10.1039/c2cp23636bPhysical Chemistry Chemical Physics1451596 ...
Wu, G., Li, W., Feng, Y.P., Guotao Wu, Wen Li, Feng, Yuan Ping, Li, Wen, Ping Chen, Xiong, Zhitao, Chen, P., Chen, Ping, Xiong, Z., Zhitao Xiong, Wu, Guotao, 陈萍, Yuan Ping Feng   +15 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Modeling solvation effects on absorption and fluorescence spectra of indole in aqueous solution. [PDF]

J Chem Phys, 2021
Abou-Hatab S, Carnevale V, Matsika S.
europepmc   +1 more source