Results 71 to 80 of about 2,312 (290)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

open access: yesFEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Chilling requirement of ten peach cultivars estimated by different models.

open access: yes, 2022
.The adaptation of a temperate climate fruit cultivar to a certain area depends mainly on its chilling requirement and the chilling accumulation in such places. Several attempts have been made to estimate these two conditions, using different models. The
RASEIRA, M. C. B.   +5 more
core  

Effects of constant and alternating temperatures on breaking dormancy of Peach (Prunus persica L.) seeds

open access: yesRevue Marocaine des Sciences Agronomiques et Vétérinaires, 1995
The effects of constant stratification temperatures on breaking peach seed dormancy were evaluated using seed populations with various chilling requirements. Germination at 20"C increased steadily with stratification time. Sorne low chilling populations
A. Mahhou, F. G. Dennis
doaj  

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Accumulated chilling hours during endodormancy impact blooming and fruit shape development in peach (Prunus persica L.)

open access: yesJournal of Integrative Agriculture, 2016
Winter chill is essential for the growth and development of deciduous species. To understand the relationship between accumulated chilling hours during endodormancy and blooming and fruit shape development, we controlled chilling hours and investigated ...
Yong LI   +6 more
doaj   +1 more source

Genetic Dissection of Bloom Time in Low Chilling Sweet Cherry (Prunus avium L.) Using a Multi-Family QTL Approach

open access: yesFrontiers in Plant Science, 2020
Bloom time in sweet cherry (Prunus avium L.) is a highly heritable trait that varies between genotypes and depends on the environmental conditions.
Alejandro Calle   +5 more
doaj   +1 more source

Nut crop yield records show that budbreak-based chilling requirements may not reflect yield decline chill thresholds

open access: yes, 2015
Warming winters due to climate change may critically affect temperate tree species. Insufficiently cold winters are thought to result in fewer viable flower buds and the subsequent development of fewer fruits or nuts, decreasing the yield of an orchard ...
Dejong, Theodore M.   +17 more
core   +1 more source

Chilling and heat requirements of apricot cultivars for flowering

open access: yesEnvironmental and Experimental Botany, 2007
Peer ...
Ruiz, David   +2 more
openaire   +2 more sources

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