Results 71 to 80 of about 61,204 (282)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Influence of exogenous gibberellin (GA3) on fruit quality in different peach varieties (strains) [PDF]

Guoshu xuebao
[Objective] Exogenous GA3 is widely used to regulate fruit growth and development, which is closely related to fruit quality. The sensitivity of exogenous GA3 varies among different fruit tree species. The aim of this study was to investigate the effects
CHEN Hong, DU Jinhua, MA Ruijuan, ZHANG Yuanyuan, DING Hui, ZHANG Binbin, YU Mingliang   +6 more
doaj   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

From Memphis, with Love: A Model to Protect Protesters in the Age of Surveillance [PDF]

, 2019
In 1978, after two years of contentious litigation, the City of Memphis entered into a unique agreement with its citizens: it signed a consent decree, stipulating that it would halt its interference with First Amendment–protected activities.
Bara, Farrah
core   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Assessment of agroclimatic suitability of apple orchards in Himachal Pradesh under changing climate

Journal of Agrometeorology, 2017
Apple crop require adequate chilling for breaking its winter dormancy for a usual spring growth. In this study, suitability of apple production was assessed by using GIS based UTAH chill unit model for tactical and strategic adaptation options under ...
JYOTI SINGH, N. R. PATEL
doaj   +1 more source

Cystic Ovarian Teratoma: Clinical and Radiographic Insights Into Chronic Pelvic Pain

Aging and Cancer, EarlyView.
An incidental benign mature cystic ovarian teratoma on imaging and discuss common imaging modalities. ABSTRACT In this case report, we present the clinical course of an 88‐year‐old woman with chronic pelvic pain attributed to an incidental benign mature cystic ovarian teratoma on imaging and discuss common imaging modalities and discussions with ...
Hashim U. Ali, Fahad H. Malik, Mohammad M. Zia, Ashar Ahmed, Mahfujul Z. Haque, Masood Siddiqui   +5 more
wiley   +1 more source

Growing apple (Malus domestica) under tropical mountain climate conditions in Northern Ethiopia [PDF]

, 2010
Lack of effective chilling during the dormant season is one of the major problems when apples are growing under a tropical climate. We evaluated the response of different apple cultivars (Golden Delicious, Gala, Fuji, Granny Smith and Jonagold) grown on ...
ALEMTSEHAY TSEGAY, DEREJE ASHEBIR, DIRK RAES, FEKADU WONDUMAGEGNEHEU, Gilreath, HAILEMARIAM TEKIE, Hauagge, Jacobs, JAN NYSSEN, JEAN POESEN, JOZEF DECKERS, Labuschagne, MINTESINOT BEHAILU, MITIKU HAILE, Njuguna, Osborne, Rice, Richardson, Shaultout, TOM DECKERS, Tromp, Webster, Willett, WUBETU BIHON   +23 more
core   +1 more source

Exposure to cold temperature affects the spring phenology of Alaskan deciduous vegetation types

Environmental Research Letters, 2020
Temperature is a dominant factor driving arctic and boreal ecosystem phenology, including leaf budburst and gross primary production (GPP) onset in Alaskan spring.
Mingjie Shi, Nicholas C Parazoo, Su-Jong Jeong, Leah Birch, Peter Lawrence, Eugenie S Euskirchen, Charles E Miller   +6 more
doaj   +1 more source

Expert consensus on research paradigms and technological layout for clinical priority diseases in traditional Chinese medicine: Rheumatoid arthritis

Advanced Chinese Medicine, EarlyView.
Abstract Rheumatoid arthritis (RA) is a chronic, highly disabling autoimmune disease. Although modern medical treatments have made progress, challenges such as suboptimal efficacy, relapse, difficulties in comorbidity management, and side effects persist.
Dier Jin, Jinping Wang, Zihan Wang, Mei Mo, Xiaojing Liu, Nan Zhang, Xiaoxiao Zhang, Qingwen Tao, Yuan Xu   +8 more
wiley   +1 more source